New functional amorphous calcium phosphate nanocomposites by enzyme-assisted biomineralization

Ee Taek Hwang, Rameshwar Tatavarty, Jinyang Chung, Man Bock Gu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the present study, we report on enzyme-assisted formation of biomineralized amorphous calcium phosphate nanocomposites (ACP-NCs). About 100-200 nm sizes of the spherical porous enzyme-assisted ACP-NCs were successfully synthesized via double reverse microemulsion, but no ACP-NCs formed without the enzyme. It is believed that the enzyme was used as an organic template or additive that could regulate the biomineralization process. The enzyme-assisted ACP-NCs were well characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, dynamic light scattering, and Brunauer-Emmett-Teller (BET) criteria. The BET surface area, total pore volume, pore size from adsorption, and pore size from desorption of the ACP-NCs were 163 m2 g-1 or 0.37 cm3 g -1, 8.87 nm, and 7.48 nm, respectively. The enzyme-assisted ACP-NCs retained about 43% of the catalytic activity of free carboxyl esterase. Furthermore, they preserved their bioactivity even after the 10th reuse and were stable over 10 days even under a stringent shaking conditions. The reported method paves the way for novel biomineralization via enzyme molecules to form functional enzymes containing nanocomposites.

Original languageEnglish
Pages (from-to)532-537
Number of pages6
JournalACS Applied Materials and Interfaces
Volume5
Issue number3
DOIs
Publication statusPublished - 2013 Feb 13

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Biomineralization
Nanocomposites
Calcium phosphate
Enzymes
Pore size
amorphous calcium phosphate
Microemulsions
Dynamic light scattering
Esterases
Bioactivity
Transmission Electron Microscopy
X-Ray Diffraction
Electron Scanning Microscopy
Adsorption
Catalyst activity
Desorption
Transmission electron microscopy
X ray diffraction
Scanning electron microscopy
Molecules

Keywords

  • amorphous calcium phosphate
  • bioinorganic nanocomposites
  • enzyme
  • enzyme assisted biomineralization
  • enzyme reuse
  • functional nanocomposites

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

New functional amorphous calcium phosphate nanocomposites by enzyme-assisted biomineralization. / Hwang, Ee Taek; Tatavarty, Rameshwar; Chung, Jinyang; Gu, Man Bock.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 3, 13.02.2013, p. 532-537.

Research output: Contribution to journalArticle

Hwang, Ee Taek ; Tatavarty, Rameshwar ; Chung, Jinyang ; Gu, Man Bock. / New functional amorphous calcium phosphate nanocomposites by enzyme-assisted biomineralization. In: ACS Applied Materials and Interfaces. 2013 ; Vol. 5, No. 3. pp. 532-537.
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