New method of manganese-enhanced magnetic resonance imaging (MEMRI) for rat brain research

Keun Yeong Jeong, Chulhyun Lee, Jee Hyun Cho, Ji Hyuk Kang, Heung Sik Na

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Manganese (Mn2+)-enhanced MRI (MEMRI) is known to provide insight into functional and anatomical biology. However, this method, which uses Mn2+ as a MRI-detectable contrast agent, has drawbacks such as the toxicity to cells beyond a certain level of Mn2+. In this study, we attempt to determine a new method of ICV administration, the optimal concentration of administered Mn2+ and the optimal MEMRI acquisition time following administration. Male Sprague-Dawley rats were used in the following experimental sessions: (1) intracerebroventricular (ICV) cannula implantation in the region of the cisterna magna, (2) serial dilution of MnCl2 (20-80 mM), (3) ICV administration of MnCl2 through the cannula, and (4) T1-weighted MRI measurements. We confirmed that cannula implantation in the region of the cisterna magna was a new ICV injection method for the administration of a contrast agent. The optimal concentration for MEMRI was 20/50 mM/μl of MnCl2. The MEMRI data acquired at different time points indicate that most signal enhancement is maintained during 14-48 h after contrast agent injection, and 24 h was the optimal time to acquire images of the rat brain. The present study offers optimized parameters for contrast agent injection that would be a good basis for studies using MEMRI to research the rat brain.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalExperimental Animals
Volume61
Issue number2
DOIs
Publication statusPublished - 2012 Apr 25

Fingerprint

Magnetic resonance
Manganese
magnetic resonance imaging
manganese
Rats
Brain
Contrast Media
Magnetic Resonance Imaging
brain
Imaging techniques
cannulas
rats
Cisterna Magna
Research
injection
Injections
methodology
Dilution
Sprague Dawley Rats
Toxicity

Keywords

  • Cannula implantation
  • Intracerebroventricular injection
  • Manganese-enhanced MRI
  • Mn
  • Rat brain

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

New method of manganese-enhanced magnetic resonance imaging (MEMRI) for rat brain research. / Jeong, Keun Yeong; Lee, Chulhyun; Cho, Jee Hyun; Kang, Ji Hyuk; Na, Heung Sik.

In: Experimental Animals, Vol. 61, No. 2, 25.04.2012, p. 157-164.

Research output: Contribution to journalArticle

Jeong, Keun Yeong ; Lee, Chulhyun ; Cho, Jee Hyun ; Kang, Ji Hyuk ; Na, Heung Sik. / New method of manganese-enhanced magnetic resonance imaging (MEMRI) for rat brain research. In: Experimental Animals. 2012 ; Vol. 61, No. 2. pp. 157-164.
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