New response surface approach to optimize medium composition for production of bacteriocin by Lactobacillus acidophilus ATCC 4356

Sungsue Rheem, Sejong Oh, Kyoung Sik Han, Jee Young Imm, Sae Hun Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The objective of this study was to optimize medium composition of initial pH, tryptone, glucose, yeast extract, and mineral mixture for production of bacteriocin by Lactobacillus acidophilus ATCC 4356, using response surface methodology. A response surface approach including new statistical and plotting methods was employed for design and analysis of the experiment. An interiorly augmented central composite design was used as an experimental design. A normal-distribution log-link generalized linear model based on a subset fourth-order polynomial (R2=0.94, Mean Error Deviance=0.0065) was used as an analysis model. This model was statistically superior to the full second-order polynomial-based generalized linear model (R2=0.80, Mean Error Deviance= 0.0140). Nonlinear programming determined the optimum composition of the medium as initial pH 6.35, tryptone 1.21%, glucose 0.9%, yeast extract 0.65%, and mineral mixture 1.17%. A validation experiment confirmed that the optimized medium was comparable to the MRS medium in bacteriocin production, having the advantage of economy and practicality.

Original languageEnglish
Pages (from-to)449-456
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume12
Issue number3
Publication statusPublished - 2002 Jan 1

Fingerprint

Lactobacillus acidophilus
Bacteriocins
Minerals
Linear Models
Yeasts
Glucose
Normal Distribution
Chemical analysis
Yeast
Research Design
Polynomials
Nonlinear programming
Normal distribution
Set theory
Design of experiments
Experiments
tryptones
Composite materials

Keywords

  • Bacteriocin
  • Lactobacillus acidophilus
  • Optimization
  • Response surface methodology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

New response surface approach to optimize medium composition for production of bacteriocin by Lactobacillus acidophilus ATCC 4356. / Rheem, Sungsue; Oh, Sejong; Han, Kyoung Sik; Imm, Jee Young; Kim, Sae Hun.

In: Journal of Microbiology and Biotechnology, Vol. 12, No. 3, 01.01.2002, p. 449-456.

Research output: Contribution to journalArticle

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