New role of human ribosomal protein S3: Regulation of cell cycle via phosphorylation by cyclin-dependent kinase 2

Se Hee Han, Ji Hyung Chung, Joon Kim, Key Sun Kim, Ye Sun Han

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Human ribosomal protein S3 (hRpS3) is a component of the 40S ribosomal subunit that associated in protein synthesis. hRpS3 has additional ribosomal functions such as DNA repair, transcription, metastasis, and apoptosis via interaction with numerous signaling molecules and has different modifications. Cyclin-dependent kinases (CDKs) are heterodimeric serine/threonine protein kinases that regulate cell cycle progression. Among its members, the Cdk1-cyclin B complex is known to control cell progression in the G2/M phase, while Cdk2-cyclin E/A complexes function in G1/S and S/G2 transition. In our previous study, we observed interaction between hRpS3 and Cdk1. The present study investigated the interaction between hRpS3 and Cdk2. Cdk2 phosphorylated hRps3 at amino acid residues S6 and T221 during the S-phase. Furthermore, hRpS3 knockdown delayed cell cycle progression by modulating the expression of cell cycle-related proteins, including cyclin B1 and cyclin E1. These findings suggest that hRpS3 is involved in Cdk2-mediated cell cycle regulation.

Original languageEnglish
Pages (from-to)3681-3687
Number of pages7
JournalOncology Letters
Volume13
Issue number5
DOIs
Publication statusPublished - 2017 May

Keywords

  • Cell cycle regulation
  • Cyclin-dependent kinase 2
  • Human ribosomal protein S3
  • In vitro kinase assay
  • Protein-protein interaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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