New Tool for Rapid and Accurate Detection of Interleukin-2 and Soluble Interleukin-2 Receptor α in Cancer Diagnosis Using a Bioresponsive Microgel and Multivalent Protein Binding

Hae Min Yang, Bora Yim, Byung Hyun Lee, Yongdoo Park, Young Gyu Kim, Jongseong Kim, Dongwon Yoo

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-2 (IL-2) and its α receptor in soluble form (sIL-2Rα) are considered biomarkers for cancers and immune-related diseases. Enzyme-linked immunosorbent assay is the most common method used to evaluate biomarkers in clinical practice; it is precise but time-consuming and involves complicated procedures. Here, we have developed a rapid yet accurate modality for cancer diagnosis that enables on-site evaluation of cancer markers, that is, IL-2 and sIL-2Rα, without complicated pretreatment of cancer patient-derived blood samples. Surface plasmon resonance and bioresponsive microgels conjugated with IL-2 receptors, that is, IL-2Rβ and IL-2Rγ, were utilized to measure IL-2 and sIL-2Rα levels via multivalent protein binding (MPB) between the ligands and their receptors. Our results showed that this novel method enables us to perform cancer diagnosis with a 1000-fold dilution of serum in 10 min. The advantage of MPB-based cancer diagnosis originates from its great selectivity for a target molecule and tolerance to a myriad of nonspecific substances in serum, which allows on-site clinical evaluation. Importantly, our finding implies that MPB-based cancer diagnosis provides a new paradigm not only for improving cancer treatment but also for evaluating a target molecule in unpurified and complex solutions such as blood.

Original languageEnglish
Pages (from-to)33782-33789
Number of pages8
JournalACS Applied Materials and Interfaces
Volume13
Issue number29
DOIs
Publication statusPublished - 2021 Jul 28

Keywords

  • biosensor
  • cancer diagnosis
  • hydrogel
  • label-free
  • multivalent protein binding
  • rapid detection
  • surface plasmon resonance

ASJC Scopus subject areas

  • Materials Science(all)

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