Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping

Yanxiang Deng, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present next generation microfluidic deformability cytometry (NG-DC) capable of analyzing single-cell deformability close to real-time and in a fully automated manner at 2,000 cells/s. NG-DC is comprised of four processes: (1) Cell injection: cell suspensions are injected into a microchannel through a pressure controller, (2) Cell alignment and spacing: randomly incoming cells form a singlestream with controlled spacing by fluid inertia, (3) Cell stretch: cells deforms upon collision at a T-junction, and (4) Data processing: images of cell elongation motion are captured, analyzed, and displayed close to real-time. Through NG-DC, various cancer cell lines have been analyzed.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages148-149
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Fingerprint

Formability
Throughput
Microfluidics
Microchannels
Elongation
Cells
Controllers
Fluids

Keywords

  • Cell mechanophenotyping
  • Inertial microfluidics
  • Single-cell analysis

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Deng, Y., & Chung, A. (2016). Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 148-149). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Next generation deformability cytometry : Fully automated, high-throughput and near real-time cell mechanotyping. / Deng, Yanxiang; Chung, Aram.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 148-149 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Deng, Y & Chung, A 2016, Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 148-149, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.
Deng Y, Chung A. Next generation deformability cytometry: Fully automated, high-throughput and near real-time cell mechanotyping. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 148-149. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
Deng, Yanxiang ; Chung, Aram. / Next generation deformability cytometry : Fully automated, high-throughput and near real-time cell mechanotyping. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 148-149 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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