NF-κB activates fibronectin gene expression in rat hepatocytes

Byung Heon Lee, Seung Yoon Park, Kae Bok Kang, Rang Woon Park, In-San Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Fibronectin (FN) plays a role in various biological processes such as fibrosis and tumor metastasis. In this study, we investigated the regulation of FN gene expression by NF-κB transcription factor. Transient expression of NF-κB p65 increased FN promoter activity in rat hepatocytes. Deletion analysis of FN promoter localized the NF-κB-responsive region at the position between -1214 and -1126. Mutations in a putative NF-κB element (5′-GAGAATTTCC-3′) at -1180 blocked most of the p65-induced promoter activity. Electromobility shift assays showed that the expression of p65 induced the binding of the p65/p65 homodimer to the NF-κB site at -1180. Stably p65-expressing cells showed increase of promoter activity, FN protein, and its mRNA levels over control cells. Furthermore, treatment of cells with interleukin-1β, a NF-κB-stimulating cytokine, also increased promoter activity, FN production, and mRNA levels. These results show that NF-κB activates FN gene expression by binding to the responsive element at -1180 as the p65/p65 homodimer in rat hepatocytes.

Original languageEnglish
Pages (from-to)1218-1224
Number of pages7
JournalBiochemical and biophysical research communications
Volume297
Issue number5
DOIs
Publication statusPublished - 2002 Nov 6
Externally publishedYes

Fingerprint

Fibronectins
Gene expression
Rats
Hepatocytes
Gene Expression
Biological Phenomena
Messenger RNA
Gene Expression Regulation
Interleukin-1
Tumors
Assays
Fibrosis
Transcription Factors
Cells
Cytokines
Neoplasm Metastasis
Mutation
Neoplasms
Proteins

Keywords

  • Fibronectin
  • Hepatocyte
  • NF-κB

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

NF-κB activates fibronectin gene expression in rat hepatocytes. / Lee, Byung Heon; Park, Seung Yoon; Kang, Kae Bok; Park, Rang Woon; Kim, In-San.

In: Biochemical and biophysical research communications, Vol. 297, No. 5, 06.11.2002, p. 1218-1224.

Research output: Contribution to journalArticle

Lee, Byung Heon ; Park, Seung Yoon ; Kang, Kae Bok ; Park, Rang Woon ; Kim, In-San. / NF-κB activates fibronectin gene expression in rat hepatocytes. In: Biochemical and biophysical research communications. 2002 ; Vol. 297, No. 5. pp. 1218-1224.
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