NFIB induces functional astrocytes from human pluripotent stem cell-derived neural precursor cells mimicking in vivo astrogliogenesis

Gyu Bum Yeon, Won Ho Shin, Seo Hyun Yoo, Dongyun Kim, Byeong Min Jeon, Won Ung Park, Yeonju Bae, Jae-Yong Park, Seungkwon You, Dokyun Na, Dae Sung Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to generate astrocytes from human pluripotent stem cells (hPSCs) offers a promising cellular model to study the development and physiology of human astrocytes. The extant methods for generating functional astrocytes required long culture periods and there remained much ambiguity on whether such paradigms follow the innate developmental program. In this report, we provided an efficient and rapid method for generating physiologically functional astrocytes from hPSCs. Overexpressing the nuclear factor IB in hPSC-derived neural precursor cells induced a highly enriched astrocyte population in 2 weeks. RNA sequencing and functional analyses demonstrated progressive transcriptomic and physiological changes in the cells, resembling in vivo astrocyte development. Further analyses substantiated previous results and established the MAPK pathway necessary for astrocyte differentiation. Hence, this differentiation paradigm provides a prospective in vitro model for human astrogliogenesis studies and the pathophysiology of neurological diseases concerning astrocytes.

Original languageEnglish
JournalJournal of Cellular Physiology
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • astrocytes
  • astrogliogenesis
  • developmental mechanisms
  • functional maturation
  • human pluripotent stem cells
  • neural precursor cells
  • NFIB

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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