Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance

Sung Un Huh, In Ju Lee, Byung Kook Ham, Kyung Hee Paek

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1- and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalMolecules and Cells
Volume34
Issue number1
DOIs
Publication statusPublished - 2012 Jul 1

Fingerprint

Ferredoxins
Tobacco
Cucumovirus
Fungal Proteins
Salinity
Gene Silencing
Chloroplasts
Gene Library
Cysteine
Proteins
Viruses

Keywords

  • Cucumber mosaic virus
  • Nicotiana tabacum
  • NtTfd1
  • Tsip1
  • VIGS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance. / Huh, Sung Un; Lee, In Ju; Ham, Byung Kook; Paek, Kyung Hee.

In: Molecules and Cells, Vol. 34, No. 1, 01.07.2012, p. 43-52.

Research output: Contribution to journalArticle

Huh, Sung Un ; Lee, In Ju ; Ham, Byung Kook ; Paek, Kyung Hee. / Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance. In: Molecules and Cells. 2012 ; Vol. 34, No. 1. pp. 43-52.
@article{e33ef0d37e014084bd676bc1943cf99a,
title = "Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance",
abstract = "Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1- and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.",
keywords = "Cucumber mosaic virus, Nicotiana tabacum, NtTfd1, Tsip1, VIGS",
author = "Huh, {Sung Un} and Lee, {In Ju} and Ham, {Byung Kook} and Paek, {Kyung Hee}",
year = "2012",
month = "7",
day = "1",
doi = "10.1007/s10059-012-0066-6",
language = "English",
volume = "34",
pages = "43--52",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

TY - JOUR

T1 - Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance

AU - Huh, Sung Un

AU - Lee, In Ju

AU - Ham, Byung Kook

AU - Paek, Kyung Hee

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1- and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.

AB - Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1- and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.

KW - Cucumber mosaic virus

KW - Nicotiana tabacum

KW - NtTfd1

KW - Tsip1

KW - VIGS

UR - http://www.scopus.com/inward/record.url?scp=84864488258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864488258&partnerID=8YFLogxK

U2 - 10.1007/s10059-012-0066-6

DO - 10.1007/s10059-012-0066-6

M3 - Article

VL - 34

SP - 43

EP - 52

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -