Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts

Jae Eun Choi, Ji Na Kim, Sang Hoon Jeong, Sang Wook Son

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3 Citations (Scopus)

Abstract

Background: Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF. Methods: Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure. Results: Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts. Conclusion: In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.

Original languageEnglish
Pages (from-to)158-161
Number of pages4
JournalInternational Journal of Dermatology
Volume49
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

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ASJC Scopus subject areas

  • Dermatology

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