Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons

Ottavio Arancio; Michael Kiebler; C. Justin Lee; Varda Lev-Ram; Roger Y. Tsien; Eric R. Kandel; Robert D. Hawkins

doi:10.1016/S0092-8674(00)81797-3

Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons

Ottavio Arancio, Michael Kiebler, C. Justin Lee, Varda Lev-Ram, Roger Y. Tsien, Eric R. Kandel, Robert D. Hawkins

KU-KIST Graduate School of Converging Science and Technology

Research output: Contribution to journal › Article

306 Citations (Scopus)

Abstract

Nitric oxide (NO) has been proposed to act as a retrograde messenger during long-term potentiation (LTP) in the CA1 region of hippocampus, but the inaccessibility of the presynaptic terminal has prevented a definitive test of this hypothesis. Because both sides of the synapse are accessible in cultured hippocampal neurons, we have used this preparation to investigate the role of NO. We examined LTP following intra- or extracellular application of an NO scavanger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that releases NO only upon photolysis with UV light. Our results indicate that NO is produced in the postsynaptic neuron, travels through the extracellular space, and acts directly in the presynaptic neuron to produce long-term potentiation, supporting the hypothesis that NO acts as a retrograde messenger during LTP.

Original language	English
Pages (from-to)	1025-1035
Number of pages	11
Journal	Cell
Volume	87
Issue number	6
DOIs	https://doi.org/10.1016/S0092-8674(00)81797-3
Publication status	Published - 1996 Dec 13

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Arancio, O., Kiebler, M., Lee, C. J., Lev-Ram, V., Tsien, R. Y., Kandel, E. R., & Hawkins, R. D. (1996). Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons. Cell, 87(6), 1025-1035. https://doi.org/10.1016/S0092-8674(00)81797-3

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abstract = "Nitric oxide (NO) has been proposed to act as a retrograde messenger during long-term potentiation (LTP) in the CA1 region of hippocampus, but the inaccessibility of the presynaptic terminal has prevented a definitive test of this hypothesis. Because both sides of the synapse are accessible in cultured hippocampal neurons, we have used this preparation to investigate the role of NO. We examined LTP following intra- or extracellular application of an NO scavanger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that releases NO only upon photolysis with UV light. Our results indicate that NO is produced in the postsynaptic neuron, travels through the extracellular space, and acts directly in the presynaptic neuron to produce long-term potentiation, supporting the hypothesis that NO acts as a retrograde messenger during LTP.",

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AU - Arancio, Ottavio

AU - Kiebler, Michael

AU - Lee, C. Justin

AU - Lev-Ram, Varda

AU - Tsien, Roger Y.

AU - Kandel, Eric R.

AU - Hawkins, Robert D.

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