Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons

Ottavio Arancio, Michael Kiebler, Changjoon Lee, Varda Lev-Ram, Roger Y. Tsien, Eric R. Kandel, Robert D. Hawkins

Research output: Contribution to journalArticle

299 Citations (Scopus)

Abstract

Nitric oxide (NO) has been proposed to act as a retrograde messenger during long-term potentiation (LTP) in the CA1 region of hippocampus, but the inaccessibility of the presynaptic terminal has prevented a definitive test of this hypothesis. Because both sides of the synapse are accessible in cultured hippocampal neurons, we have used this preparation to investigate the role of NO. We examined LTP following intra- or extracellular application of an NO scavanger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that releases NO only upon photolysis with UV light. Our results indicate that NO is produced in the postsynaptic neuron, travels through the extracellular space, and acts directly in the presynaptic neuron to produce long-term potentiation, supporting the hypothesis that NO acts as a retrograde messenger during LTP.

Original languageEnglish
Pages (from-to)1025-1035
Number of pages11
JournalCell
Volume87
Issue number6
DOIs
Publication statusPublished - 1996 Dec 13
Externally publishedYes

Fingerprint

Long-Term Potentiation
Neurons
Nitric Oxide
Nitric Oxide Donors
Photolysis
Presynaptic Terminals
Extracellular Space
Ultraviolet Rays
Nitric Oxide Synthase
Ultraviolet radiation
Synapses
Hippocampus
Membranes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Arancio, O., Kiebler, M., Lee, C., Lev-Ram, V., Tsien, R. Y., Kandel, E. R., & Hawkins, R. D. (1996). Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons. Cell, 87(6), 1025-1035. https://doi.org/10.1016/S0092-8674(00)81797-3

Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons. / Arancio, Ottavio; Kiebler, Michael; Lee, Changjoon; Lev-Ram, Varda; Tsien, Roger Y.; Kandel, Eric R.; Hawkins, Robert D.

In: Cell, Vol. 87, No. 6, 13.12.1996, p. 1025-1035.

Research output: Contribution to journalArticle

Arancio, O, Kiebler, M, Lee, C, Lev-Ram, V, Tsien, RY, Kandel, ER & Hawkins, RD 1996, 'Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons', Cell, vol. 87, no. 6, pp. 1025-1035. https://doi.org/10.1016/S0092-8674(00)81797-3
Arancio, Ottavio ; Kiebler, Michael ; Lee, Changjoon ; Lev-Ram, Varda ; Tsien, Roger Y. ; Kandel, Eric R. ; Hawkins, Robert D. / Nitric oxide acts directly in the presynaptic neuron to produce long- term potentiation in cultured hippocampal neurons. In: Cell. 1996 ; Vol. 87, No. 6. pp. 1025-1035.
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