Nitric oxide inhibits an interaction between JNK1 and c-Jun through nitrosylation

Hee Sae Park, Jung Soon Mo, Eui Ju Choi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nitric oxide (NO) has been shown to negatively regulate c-Jun N-terminal kinase (JNK) through S-nitrosylation. Here, we show that disruption of an interaction between JNK and its substrate c-Jun is an important mechanism underlying the NO-mediated inhibition of JNK signaling. Endogenous NO, which was generated by interferon-γ treatment, suppressed anisomycin-stimulated JNK activity in microglial BV-2 cells. The interferon-γ-induced suppression of JNK1 activation in BV-2 cells was prevented completely by treatment with NG-nitro-l-arginine, an inhibitor of NO synthase. A NO donor S-nitro-N-acetyl-dl-penicillamine (SNAP) inhibited JNK activity in vitro, and this inhibition was reversed by a thiol-reducing agent, dithiothreitol. Nitric oxide disrupts a physical interaction between JNK and its substrate c-Jun both in vitro and in intact cells without affecting an interaction between SEK1 and JNK. Collectively, our results suggest that the inhibition of the interaction between JNK and c-Jun may be an integral part of the mechanism underlying the negative regulation of the JNK signaling pathway by NO.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalBiochemical and biophysical research communications
Volume351
Issue number1
DOIs
Publication statusPublished - 2006 Dec 8

Keywords

  • JNK
  • NO
  • Nitrosylation
  • c-Jun

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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