Nitric oxide modulates the c-Jun N-terminal kinase/stress-activated protein kinase activity through activating c-Jun N-terminal kinase kinase

Hanshin Kim, Jaekyung Shim, Pyung Lim Han, Eui Ju Choi

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Abstract

Nitric oxide is a signaling molecule that has a broad range of physiological functions, including neurotransmission, macrophage activation, and vasodilation. The mechanism by which nitric oxide regulates signal transduction mediating diverse biological activities is not fully understood, however. Here, we demonstrate that nitric oxide induced the stimulation of c- Jun NH2-terminal kinase (JNK)/stress-activated protein kinase (SAPK) in intact cells. Exposure of cultured HEK293 cells to sodium nitroprusside, a nitric oxide releasing agent, resulted in the stimulation of JNK1 activity. The sodium nitroprusside-induced stimulation of JNK1 activity was abolished by treatment of cells with N-acetylcysteine. Nitric oxide production from HEK293 cells ectopically expressing nitric oxide synthases resulted in the stimulation of JNK1 activity, while JNK1 stimulation in nitric oxide synthase-overexpressing cells was abrogated by a nitric oxide synthase inhibitor, N(G)-nitro-L-arginine. Furthermore, exposure of cells to sodium nitroprusside resulted in the stimulation of JNK kinase (JNKK1/SEK1). Taken together, our data suggest that nitric oxide modulates the JNK activity through activating JNKK1/SEK1.

Original languageEnglish
Pages (from-to)13677-13681
Number of pages5
JournalBiochemistry
Volume36
Issue number44
DOIs
Publication statusPublished - 1997 Nov 4

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JNK Mitogen-Activated Protein Kinases
Heat-Shock Proteins
Protein Kinases
Nitric Oxide
Phosphotransferases
Nitroprusside
Nitric Oxide Synthase
HEK293 Cells
Signal transduction
Macrophage Activation
Macrophages
Acetylcysteine
Bioactivity
Vasodilation
Synaptic Transmission
Arginine
Cultured Cells
Signal Transduction
Chemical activation
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nitric oxide modulates the c-Jun N-terminal kinase/stress-activated protein kinase activity through activating c-Jun N-terminal kinase kinase. / Kim, Hanshin; Shim, Jaekyung; Han, Pyung Lim; Choi, Eui Ju.

In: Biochemistry, Vol. 36, No. 44, 04.11.1997, p. 13677-13681.

Research output: Contribution to journalArticle

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