Nitrifying Bacterial Community Structure of a Full-Scale Integrated Fixed-Film Activated Sludge Process as Investigated by Pyrosequencing

Taek Seung Kim, Han Shin Kim, Soondong Kwon, Hee-Deung Park

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Nitrifying bacterial community structures of suspended and attached biomasses in a full-scale integrated fixed-film activated sludge process were investigated by analyzing 16S rRNA gene sequences obtained from pyrosequencing. The suspended biomass had a higher number of ammonia-oxidizing bacterial sequences (0.8% of total sequences) than the attached biomass (0.07%), although most of the sequences were within the Nitrosomonas oligotropha lineage in both biomasses. Nitrospira-like nitrite-oxidizing bacterial sequences were retrieved in the suspended biomass (0.06%), not in the attached biomass, whereas the existence of Nitrobacter-like sequences was not evident. The suspended biomass had higher nitrification activity (1.13 mg N/TSS/h) than the attached biomass (0.07 mg N/TSS/h). Overall, the results made it possible to conclude the importance of the suspended biomass, rather than the attached biomass, in nitrification in the wastewater treatment process studied.

Original languageEnglish
Pages (from-to)293-298
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume21
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Fingerprint

Bacterial Structures
Sewage
Biomass
Nitrification
Nitrobacter
Nitrosomonas
Nitrites
Waste Water
rRNA Genes
Ammonia

Keywords

  • Activated sludge
  • AOB
  • IFAS
  • NOB
  • Pyrosequencing

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Nitrifying Bacterial Community Structure of a Full-Scale Integrated Fixed-Film Activated Sludge Process as Investigated by Pyrosequencing. / Kim, Taek Seung; Kim, Han Shin; Kwon, Soondong; Park, Hee-Deung.

In: Journal of Microbiology and Biotechnology, Vol. 21, No. 3, 01.03.2011, p. 293-298.

Research output: Contribution to journalArticle

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