Nitrosoreductase-Like Nanocatalyst for Ultrasensitive and Stable Biosensing

Ponnusamy Nandhakumar, Byeongyoon Kim, Nam Sihk Lee, Young Ho Yoon, Kwangyeol Lee, Haesik Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Enzyme-like nanocatalytic reactions developed for high signal amplification in biosensors are of limited use because of their low reaction rates and/or unwanted side reactions in aqueous electrolyte solutions containing dissolved O2. Herein, we report a nitrosoreductase-like catalytic reaction, employing 4-nitroso-1-naphthol, Pd nanoparticles, and H3N-BH3, which affords a high reaction rate and minimal side reactions, enabling its use in ultrasensitive electrochemical biosensors. 4-Nitroso-1-naphthol was chosen after five hydroxy-nitro(so)arene compounds were compared in terms of high signal and low background levels. Importantly, the nanocatalytic reaction occurs without the self-hydrolysis and induction period observed in the nanocatalytic reduction of nitroarenes by NaBH4. The high signal level results from (i) fast nanocatalytic 4-nitroso-1-naphthol reduction, (ii) fast electrochemical redox cycling, and (iii) the low influence of dissolved O2. The low background level results from (i) slow direct reaction between 4-nitroso-1-naphthol and H3N-BH3, (ii) slow electrode-mediated reaction between 4-nitroso-1-naphthol and H3N-BH3, and (iii) slow electrooxidation of H3N-BH3 at electrode. When applied to the detection of parathyroid hormone, the detection limit of the newly developed biosensor was ∼0.3 pg/mL. The nitrosoreductase-like nanocatalytic reaction is highly promising for ultrasensitive and stable biosensing.

Original languageEnglish
Pages (from-to)807-813
Number of pages7
JournalAnalytical Chemistry
Volume90
Issue number1
DOIs
Publication statusPublished - 2018 Jan 2

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Biosensors
Reaction rates
Electrodes
Electrooxidation
Parathyroid Hormone
Electrolytes
Amplification
Hydrolysis
1-naphthol
Nanoparticles
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Nitrosoreductase-Like Nanocatalyst for Ultrasensitive and Stable Biosensing. / Nandhakumar, Ponnusamy; Kim, Byeongyoon; Lee, Nam Sihk; Yoon, Young Ho; Lee, Kwangyeol; Yang, Haesik.

In: Analytical Chemistry, Vol. 90, No. 1, 02.01.2018, p. 807-813.

Research output: Contribution to journalArticle

Nandhakumar, Ponnusamy ; Kim, Byeongyoon ; Lee, Nam Sihk ; Yoon, Young Ho ; Lee, Kwangyeol ; Yang, Haesik. / Nitrosoreductase-Like Nanocatalyst for Ultrasensitive and Stable Biosensing. In: Analytical Chemistry. 2018 ; Vol. 90, No. 1. pp. 807-813.
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