Niveibacterium microcysteis sp. Nov., isolated from a cyanobacterial bloom sample

Mingyeong Kang, Woojun Park

Research output: Contribution to journalArticlepeer-review

Abstract

A novel bacterial strain, named HC41T, was isolated from a cyanobacterial bloom sample and was characterized as Gram-stain-negative, rod-shaped and non-motile. According to 16S rRNA phylogenetic analyses, this strain HC41T belongs to the family Rhodocyclaceae and is most closely related to Niveibacterium umoris KACC 17062T (=MIC 2059T; 98.63 %) and Uliginosi-bacterium gangwonense 5YN10-9T (=KACC 11603T; 93.64 %). The genome size and DNA G+C content of strain HC41T were 4.8 Mbp and 64.17 mol%, respectively. Moreover, the average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain HC41T and N. umoris KACC 17062T were 81.8, 43.1 and 90.89 %, respectively. Additionally, strain HC41T exhibited weak catalase and oxidase activities and had no motility (swimming and swarming motilities). The cells grew at 11–40 °C (optimum, 30 °C), pH 5.5–8.0 (optimum, pH 7) and with 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl) in Reasoner’s 2A medium. Its major respiratory quinone was ubiquinone-8 and its major polar lipids were diphosphatidylglycerol, phosphatidyl-glycerol and phosphatidylethanolamine. Furthermore, C16: 0 and summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c; C16: 1 ω6c and/or C16: 1 ω7c) were the predominant cellular fatty acids in strain HC41T according to fatty acid methyl ester analysis. Based on its genotypic and phenotypic characteristics, strain HC41T was identified as representing a novel Niveibacterium species, for which the name Niveibacterium microcysteis sp. nov. is proposed (=KACC 22091T=DSM 111425T).

Original languageEnglish
Article number004845
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume71
Issue number7
DOIs
Publication statusPublished - 2021

Keywords

  • Cyanobacterial bloom
  • Freshwater
  • Genomics
  • Microcystis aeruginosa
  • Niveibacterium
  • Proteobacteria

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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