TY - JOUR
T1 - Niveibacterium microcysteis sp. Nov., isolated from a cyanobacterial bloom sample
AU - Kang, Mingyeong
AU - Park, Woojun
N1 - Funding Information:
This work was supported by a grant from the National Institute of Biological Resources (NIBR), which was funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108).
Publisher Copyright:
© 2021 The Authors.
PY - 2021
Y1 - 2021
N2 - A novel bacterial strain, named HC41T, was isolated from a cyanobacterial bloom sample and was characterized as Gram-stain-negative, rod-shaped and non-motile. According to 16S rRNA phylogenetic analyses, this strain HC41T belongs to the family Rhodocyclaceae and is most closely related to Niveibacterium umoris KACC 17062T (=MIC 2059T; 98.63 %) and Uliginosi-bacterium gangwonense 5YN10-9T (=KACC 11603T; 93.64 %). The genome size and DNA G+C content of strain HC41T were 4.8 Mbp and 64.17 mol%, respectively. Moreover, the average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain HC41T and N. umoris KACC 17062T were 81.8, 43.1 and 90.89 %, respectively. Additionally, strain HC41T exhibited weak catalase and oxidase activities and had no motility (swimming and swarming motilities). The cells grew at 11–40 °C (optimum, 30 °C), pH 5.5–8.0 (optimum, pH 7) and with 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl) in Reasoner’s 2A medium. Its major respiratory quinone was ubiquinone-8 and its major polar lipids were diphosphatidylglycerol, phosphatidyl-glycerol and phosphatidylethanolamine. Furthermore, C16: 0 and summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c; C16: 1 ω6c and/or C16: 1 ω7c) were the predominant cellular fatty acids in strain HC41T according to fatty acid methyl ester analysis. Based on its genotypic and phenotypic characteristics, strain HC41T was identified as representing a novel Niveibacterium species, for which the name Niveibacterium microcysteis sp. nov. is proposed (=KACC 22091T=DSM 111425T).
AB - A novel bacterial strain, named HC41T, was isolated from a cyanobacterial bloom sample and was characterized as Gram-stain-negative, rod-shaped and non-motile. According to 16S rRNA phylogenetic analyses, this strain HC41T belongs to the family Rhodocyclaceae and is most closely related to Niveibacterium umoris KACC 17062T (=MIC 2059T; 98.63 %) and Uliginosi-bacterium gangwonense 5YN10-9T (=KACC 11603T; 93.64 %). The genome size and DNA G+C content of strain HC41T were 4.8 Mbp and 64.17 mol%, respectively. Moreover, the average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain HC41T and N. umoris KACC 17062T were 81.8, 43.1 and 90.89 %, respectively. Additionally, strain HC41T exhibited weak catalase and oxidase activities and had no motility (swimming and swarming motilities). The cells grew at 11–40 °C (optimum, 30 °C), pH 5.5–8.0 (optimum, pH 7) and with 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl) in Reasoner’s 2A medium. Its major respiratory quinone was ubiquinone-8 and its major polar lipids were diphosphatidylglycerol, phosphatidyl-glycerol and phosphatidylethanolamine. Furthermore, C16: 0 and summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c; C16: 1 ω6c and/or C16: 1 ω7c) were the predominant cellular fatty acids in strain HC41T according to fatty acid methyl ester analysis. Based on its genotypic and phenotypic characteristics, strain HC41T was identified as representing a novel Niveibacterium species, for which the name Niveibacterium microcysteis sp. nov. is proposed (=KACC 22091T=DSM 111425T).
KW - Cyanobacterial bloom
KW - Freshwater
KW - Genomics
KW - Microcystis aeruginosa
KW - Niveibacterium
KW - Proteobacteria
UR - http://www.scopus.com/inward/record.url?scp=85109137784&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004845
DO - 10.1099/ijsem.0.004845
M3 - Article
C2 - 34232855
AN - SCOPUS:85109137784
SN - 1466-5026
VL - 71
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 7
M1 - 004845
ER -