NMDA receptor-mediated calcium influx plays an essential role in myoblast fusion

Kun Ho Lee, Jae Yong Park, Kyungjin Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ca 2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca 2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca 2+ imaging analysis, Ca 2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca 2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca 2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca 2+ influx.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalFEBS Letters
Volume578
Issue number1-2
DOIs
Publication statusPublished - 2004 Dec 3

Keywords

  • C2C12 myoblast
  • Ca influx
  • Muscle differentiation
  • Myoblast fusion
  • N-Methyl-D-aspartate receptor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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