NO inhibitory phytochemicals as potential anti-inflammatory agents from the twigs of Trigonostemon heterophyllus

Yaru Xi, Lijun An, Xueyuan Yang, Ziteng Song, Jie Zhang, Muhetaer Tuerhong, Da Qing Jin, Yasushi Ohizumi, Dongho Lee, Jing Xu, Yuanqiang Guo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Studies on the relationship of nitric oxide (NO) and inflammation have revealed that compounds with NO inhibitory effects are potentially useful for inflammation and related inflammatory disorders. A phytochemical investigation to obtain new NO inhibitors resulted in the isolation of two new cleistanthane diterpenoids (1 and 2) and 11 known terpenoids (3–13) from Trigonostemon heterophyllus. The structures of these terpenoids were established by analysis of their NMR, MS, and electronic circular dichroism (ECD) data. Compounds 1 and 2 possess rare 3,4-seco-cleistanthane diterpenoid skeletons. All of the isolates were evaluated biologically for their NO inhibitory effects in lipopolysaccharide (LPS)-induced murine microglial BV-2 cells and compounds 1, 6, and 8–10 showed strong NO inhibitory effects with IC 50 values less than 40 μM. Using Western blotting experiments and molecular docking, the possible mechanism of NO inhibition was investigated.

Original languageEnglish
Pages (from-to)417-424
Number of pages8
JournalBioorganic Chemistry
Volume87
DOIs
Publication statusPublished - 2019 Jun

Keywords

  • Cleistanthane diterpenoids
  • Inflammation
  • Molecular docking
  • NO inhibitors
  • Trigonostemon heterophyllus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

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  • Cite this

    Xi, Y., An, L., Yang, X., Song, Z., Zhang, J., Tuerhong, M., Jin, D. Q., Ohizumi, Y., Lee, D., Xu, J., & Guo, Y. (2019). NO inhibitory phytochemicals as potential anti-inflammatory agents from the twigs of Trigonostemon heterophyllus. Bioorganic Chemistry, 87, 417-424. https://doi.org/10.1016/j.bioorg.2019.03.029