Non-labeled detection of waterborne pathogen Cryptosporidium parvum using a polydiacetylene-based fluorescence chip

Cheon Kyo Park, Chang Duk Kang, Sang Jun Sim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A non-labeling fluorescence sensor system was developed using polydiacetylene (PDA) liposomes composed of 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a 8:2 molar ratio. The PDA liposomes were immobilized onto an amine-coated glass surface using peptide bonding between the carboxyl group of the liposome and the amine group of the glass surface. The optimum ratio of the cross linker (NHS/EDC) to PDA liposome was determined to be 50% for strong immobilization of the liposomes. Residual carboxyl groups of the PDA liposomes were selectively biotinylated, followed by sequential binding of streptavidin and biotin-antibody (bioreceptor). Finally, the performance of the PDA liposome chip was tested for detecting Cryptosporidium parvum, and yielded a detection limit of 1 × 103 oocysts/mL. From these results, it is expected that the PDA liposome chip will have high application potential for the detection of waterborne pathogens including C. Parvum.

Original languageEnglish
Pages (from-to)687-693
Number of pages7
JournalBiotechnology Journal
Volume3
Issue number5
DOIs
Publication statusPublished - 2008 May 1
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Liposomes
Fluorescence
Amines
Glass
Phosphorylcholine
Streptavidin
Oocysts
polydiacetylene
Biotin
Immobilization
Limit of Detection
Peptides
Antibodies

Keywords

  • Cryptosporidium parvum
  • Fluorescence chip
  • Immobilization
  • Liposome
  • Polydiacetylene

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Non-labeled detection of waterborne pathogen Cryptosporidium parvum using a polydiacetylene-based fluorescence chip. / Park, Cheon Kyo; Kang, Chang Duk; Sim, Sang Jun.

In: Biotechnology Journal, Vol. 3, No. 5, 01.05.2008, p. 687-693.

Research output: Contribution to journalArticle

@article{6668888ca54e42a3a39c21265c1f4438,
title = "Non-labeled detection of waterborne pathogen Cryptosporidium parvum using a polydiacetylene-based fluorescence chip",
abstract = "A non-labeling fluorescence sensor system was developed using polydiacetylene (PDA) liposomes composed of 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a 8:2 molar ratio. The PDA liposomes were immobilized onto an amine-coated glass surface using peptide bonding between the carboxyl group of the liposome and the amine group of the glass surface. The optimum ratio of the cross linker (NHS/EDC) to PDA liposome was determined to be 50{\%} for strong immobilization of the liposomes. Residual carboxyl groups of the PDA liposomes were selectively biotinylated, followed by sequential binding of streptavidin and biotin-antibody (bioreceptor). Finally, the performance of the PDA liposome chip was tested for detecting Cryptosporidium parvum, and yielded a detection limit of 1 × 103 oocysts/mL. From these results, it is expected that the PDA liposome chip will have high application potential for the detection of waterborne pathogens including C. Parvum.",
keywords = "Cryptosporidium parvum, Fluorescence chip, Immobilization, Liposome, Polydiacetylene",
author = "Park, {Cheon Kyo} and Kang, {Chang Duk} and Sim, {Sang Jun}",
year = "2008",
month = "5",
day = "1",
doi = "10.1002/biot.200700246",
language = "English",
volume = "3",
pages = "687--693",
journal = "Biotechnology Journal",
issn = "1860-6768",
publisher = "Wiley-VCH Verlag",
number = "5",

}

TY - JOUR

T1 - Non-labeled detection of waterborne pathogen Cryptosporidium parvum using a polydiacetylene-based fluorescence chip

AU - Park, Cheon Kyo

AU - Kang, Chang Duk

AU - Sim, Sang Jun

PY - 2008/5/1

Y1 - 2008/5/1

N2 - A non-labeling fluorescence sensor system was developed using polydiacetylene (PDA) liposomes composed of 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a 8:2 molar ratio. The PDA liposomes were immobilized onto an amine-coated glass surface using peptide bonding between the carboxyl group of the liposome and the amine group of the glass surface. The optimum ratio of the cross linker (NHS/EDC) to PDA liposome was determined to be 50% for strong immobilization of the liposomes. Residual carboxyl groups of the PDA liposomes were selectively biotinylated, followed by sequential binding of streptavidin and biotin-antibody (bioreceptor). Finally, the performance of the PDA liposome chip was tested for detecting Cryptosporidium parvum, and yielded a detection limit of 1 × 103 oocysts/mL. From these results, it is expected that the PDA liposome chip will have high application potential for the detection of waterborne pathogens including C. Parvum.

AB - A non-labeling fluorescence sensor system was developed using polydiacetylene (PDA) liposomes composed of 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a 8:2 molar ratio. The PDA liposomes were immobilized onto an amine-coated glass surface using peptide bonding between the carboxyl group of the liposome and the amine group of the glass surface. The optimum ratio of the cross linker (NHS/EDC) to PDA liposome was determined to be 50% for strong immobilization of the liposomes. Residual carboxyl groups of the PDA liposomes were selectively biotinylated, followed by sequential binding of streptavidin and biotin-antibody (bioreceptor). Finally, the performance of the PDA liposome chip was tested for detecting Cryptosporidium parvum, and yielded a detection limit of 1 × 103 oocysts/mL. From these results, it is expected that the PDA liposome chip will have high application potential for the detection of waterborne pathogens including C. Parvum.

KW - Cryptosporidium parvum

KW - Fluorescence chip

KW - Immobilization

KW - Liposome

KW - Polydiacetylene

UR - http://www.scopus.com/inward/record.url?scp=44949155563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949155563&partnerID=8YFLogxK

U2 - 10.1002/biot.200700246

DO - 10.1002/biot.200700246

M3 - Article

C2 - 18381618

AN - SCOPUS:44949155563

VL - 3

SP - 687

EP - 693

JO - Biotechnology Journal

JF - Biotechnology Journal

SN - 1860-6768

IS - 5

ER -