Non-labeled detection of waterborne pathogen Cryptosporidium parvum using a polydiacetylene-based fluorescence chip

Cheon Kyo Park, Chang Duk Kang, Sang Jun Sim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A non-labeling fluorescence sensor system was developed using polydiacetylene (PDA) liposomes composed of 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a 8:2 molar ratio. The PDA liposomes were immobilized onto an amine-coated glass surface using peptide bonding between the carboxyl group of the liposome and the amine group of the glass surface. The optimum ratio of the cross linker (NHS/EDC) to PDA liposome was determined to be 50% for strong immobilization of the liposomes. Residual carboxyl groups of the PDA liposomes were selectively biotinylated, followed by sequential binding of streptavidin and biotin-antibody (bioreceptor). Finally, the performance of the PDA liposome chip was tested for detecting Cryptosporidium parvum, and yielded a detection limit of 1 × 103 oocysts/mL. From these results, it is expected that the PDA liposome chip will have high application potential for the detection of waterborne pathogens including C. Parvum.

Original languageEnglish
Pages (from-to)687-693
Number of pages7
JournalBiotechnology Journal
Volume3
Issue number5
DOIs
Publication statusPublished - 2008 May 1
Externally publishedYes

Keywords

  • Cryptosporidium parvum
  • Fluorescence chip
  • Immobilization
  • Liposome
  • Polydiacetylene

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

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