Non-Photosynthetic CO2Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Seung Kyou You, Hyeon Min Park, Myeong Eun Lee, Young Jin Ko, Dong Hyeuk Hwang, Jun Won Oh, Sung Ok Han

Research output: Contribution to journalArticlepeer-review

Abstract

Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been focused on as a green strategy to convert CO2 into valuable products such as fatty acids. In this study, a CO2 utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO2 in the oleaginous yeast Yarrowia lipolytica, thereby increasing the production of fatty acids. In the recombinant strain in which the CO2 utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO2.

Original languageEnglish
Pages (from-to)11912-11918
Number of pages7
JournalJournal of agricultural and food chemistry
Volume69
Issue number40
DOIs
Publication statusPublished - 2021 Oct 13

Keywords

  • COutilization
  • Yarrowia lipolytica
  • biotin carboxylase
  • carbonic anhydrase
  • non-photosynthetic

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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