Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Hee Jin Jun, Mi Ja Chung, Sang Yeon Kim, Ho Joung Lee, Sung Joon Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.

Original languageEnglish
Pages (from-to)1805-1810
Number of pages6
JournalBiotechnology letters
Volume28
Issue number22
DOIs
Publication statusPublished - 2006 Nov

Keywords

  • Anti-atherogenic
  • Atherosclerosis
  • Endothelium/monocyte binding

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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