Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Hee Jin Jun, Mi J. Chung, Sang Yeon Kim, Hojoung Lee, Sung-Joon Lee

Research output: Contribution to journalArticle

Abstract

Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.

Original languageEnglish
Pages (from-to)1805-1810
Number of pages6
JournalBiotechnology Letters
Volume28
Issue number22
DOIs
Publication statusPublished - 2006 Nov 1

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Keywords

  • Anti-atherogenic
  • Atherosclerosis
  • Endothelium/monocyte binding

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

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