Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Hee Jin Jun; Mi J. Chung; Sang Yeon Kim; Hojoung Lee; Sung-Joon Lee

doi:10.1007/s10529-006-9165-9

Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Hee Jin Jun, Mi J. Chung, Sang Yeon Kim, Hojoung Lee, Sung-Joon Lee

Research output: Contribution to journal › Article

Abstract

Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.

Original language	English
Pages (from-to)	1805-1810
Number of pages	6
Journal	Biotechnology Letters
Volume	28
Issue number	22
DOIs	https://doi.org/10.1007/s10529-006-9165-9
Publication status	Published - 2006 Nov 1

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Keywords

Anti-atherogenic
Atherosclerosis
Endothelium/monocyte binding

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Microbiology

Cite this

Jun, H. J., Chung, M. J., Kim, S. Y., Lee, H., & Lee, S-J. (2006). Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis. Biotechnology Letters, 28(22), 1805-1810. https://doi.org/10.1007/s10529-006-9165-9

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10.1007/s10529-006-9165-9