Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Hee Jin Jun; Mi Ja Chung; Sang Yeon Kim; Ho Joung Lee; Sung Joon Lee

doi:10.1007/s10529-006-9165-9

Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Hee Jin Jun, Mi Ja Chung, Sang Yeon Kim, Ho Joung Lee, Sung Joon Lee

Research output: Contribution to journal › Article › peer-review

Abstract

Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.

Original language	English
Pages (from-to)	1805-1810
Number of pages	6
Journal	Biotechnology letters
Volume	28
Issue number	22
DOIs	https://doi.org/10.1007/s10529-006-9165-9
Publication status	Published - 2006 Nov

Keywords

Anti-atherogenic
Atherosclerosis
Endothelium/monocyte binding

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis",

abstract = "Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.",

keywords = "Anti-atherogenic, Atherosclerosis, Endothelium/monocyte binding",

author = "Jun, {Hee Jin} and Chung, {Mi Ja} and Kim, {Sang Yeon} and Lee, {Ho Joung} and Lee, {Sung Joon}",

note = "Funding Information: Acknowledgements This Study was supported by Technology Development Program of the Ministry of Agriculture and Forestry (105054-03-2-HD110) and a grant from the Biogreen 21 program, Rural Development Administration (20050401-034-749-180-02-00), Republic of Korea",

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language = "English",

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T1 - Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

AU - Jun, Hee Jin

AU - Chung, Mi Ja

AU - Kim, Sang Yeon

AU - Lee, Ho Joung

AU - Lee, Sung Joon

N1 - Funding Information: Acknowledgements This Study was supported by Technology Development Program of the Ministry of Agriculture and Forestry (105054-03-2-HD110) and a grant from the Biogreen 21 program, Rural Development Administration (20050401-034-749-180-02-00), Republic of Korea

PY - 2006/11

Y1 - 2006/11

N2 - Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.

AB - Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.

KW - Anti-atherogenic

KW - Atherosclerosis

KW - Endothelium/monocyte binding

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