Abstract
Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 1805-1810 |
| Number of pages | 6 |
| Journal | Biotechnology letters |
| Volume | 28 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 2006 Nov |
Keywords
- Anti-atherogenic
- Atherosclerosis
- Endothelium/monocyte binding
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Cite this
Jun, H. J., Chung, M. J., Kim, S. Y., Lee, H. J., & Lee, S. J. (2006). Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis. Biotechnology letters, 28(22), 1805-1810. https://doi.org/10.1007/s10529-006-9165-9