Noninvasive Diagnosis and Evaluation of Curative Surgery for Gastric Cancer by Using NMR-based Metabolomic Profiling

Jeeyoun Jung, Youngae Jung, Eun Jung Bang, Sung il Cho, You-Jin Jang, Jung-Myun Kwak, Do Hyun Ryu, Sungsoo Park, Geum Sook Hwang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Methods: A total of 154 urine samples from GC patients and healthy individuals and 30 pairs of matched tumor and normal stomach tissues were collected. Multivariate analysis was performed on urinary and tissue metabolic profiles acquired using 1H nuclear magnetic resonance and 1H high-resolution magic angle spinning spectroscopy, respectively. In addition, metabolic profiling of urine from GC patients after curative surgery was performed.

Background: Mass screening for gastric cancer (GC), particularly using endoscopy, may not be the most practical approach as a result of its high cost, lack of acceptance, and poor availability. Thus, novel markers that can be used in cost-effective diagnosis and noninvasive screening for GC are needed.

Results: Multivariate statistical analysis showed significant separation in the urinary and tissue data of GC patients and healthy individuals. The metabolites altered in the urine of GC patients were related to amino acid and lipid metabolism, consistent with changes in GC tissue. In the external validation, the presence of GC (early or advanced) from the urine model was predicted with high accuracy, which showed much higher sensitivity than carbohydrate antigen 19-9 and carcinoembryonic antigen. Furthermore, 4-hydroxyphenylacetate, alanine, phenylacetylglycine, mannitol, glycolate, and arginine levels were significantly correlated with cancer T stage and, together with hypoxanthine level, showed a recovery tendency toward healthy controls in the postoperative samples compared to the preoperative samples.

Conclusions: An urinary metabolomics approach may be useful for the effective diagnosis of GC.

Original languageEnglish
Pages (from-to)736-742
Number of pages7
JournalAnnals of Surgical Oncology
Volume21
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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Metabolomics
Stomach Neoplasms
Urine
glycolic acid
Multivariate Analysis
Costs and Cost Analysis
Mass Screening
Hypoxanthine
Metabolome
Carcinoembryonic Antigen
Mannitol
Lipid Metabolism
Alanine
Endoscopy
Arginine
Neoplasms
Spectrum Analysis
Stomach
Magnetic Resonance Spectroscopy
Carbohydrates

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Noninvasive Diagnosis and Evaluation of Curative Surgery for Gastric Cancer by Using NMR-based Metabolomic Profiling. / Jung, Jeeyoun; Jung, Youngae; Bang, Eun Jung; Cho, Sung il; Jang, You-Jin; Kwak, Jung-Myun; Ryu, Do Hyun; Park, Sungsoo; Hwang, Geum Sook.

In: Annals of Surgical Oncology, Vol. 21, No. 4, 01.01.2014, p. 736-742.

Research output: Contribution to journalArticle

Jung, Jeeyoun ; Jung, Youngae ; Bang, Eun Jung ; Cho, Sung il ; Jang, You-Jin ; Kwak, Jung-Myun ; Ryu, Do Hyun ; Park, Sungsoo ; Hwang, Geum Sook. / Noninvasive Diagnosis and Evaluation of Curative Surgery for Gastric Cancer by Using NMR-based Metabolomic Profiling. In: Annals of Surgical Oncology. 2014 ; Vol. 21, No. 4. pp. 736-742.
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