Nonsense-mediated translational repression involves exon junction complex downstream of premature translation termination codon

Hyung Chul Lee, Nara Oh, Hana Cho, Junho Choe, Yoon Ki Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Human transforming growth factor-β receptor type 2 (TGFβR2) mRNA harboring a premature translation termination codon (PTC) generated by frameshift mutation is targeted for nonsense-mediated translational repression (NMTR), rather than nonsense-mediated mRNA decay (NMD). Here we show that exon junction complex (EJC) downstream of a PTC plays an inhibitory role in translation of TGFβR2 mRNA. Translational repression by core EJC components occurs after formation of 80S ribosome complex, which is demonstrated using different types of internal ribosome entry sites (IRESes). Our findings implicate EJCs or core EJC components as negative regulators of translation.

Original languageEnglish
Pages (from-to)795-800
Number of pages6
JournalFEBS Letters
Volume584
Issue number4
DOIs
Publication statusPublished - 2010 Feb

Keywords

  • EIF4AIII
  • EJC
  • NMD
  • NMTR
  • Y14

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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