Noradrenergic neurotoxin suppresses gonadotropin-releasing hormone (GnRH) and GnRH receptor gene expression in ovariectomized and steroid-treated rats

S. S. Kang, Gi Hoon Son, Jae Young Seong, D. Choi, H. B. Kwon, C. C. Lee, K. Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present study was designed to investigate whether noradrenergic neurotransmission regulates the gene expression of gonadotropin-releasing hormone (GnRH) in the preoptic area and GnRH receptor in the pituitary. To this end, N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4, 50 mg/kg), an intraperitoneal (i.p.) injection of selective noradrenergic neurotoxin, was administered 1 h before progesterone (1 mg) treatment in ovariectomized and estradiol-treated prepubertal rats. Treatment with DSP4 effectively blocked the progesterone-induced increase in hypothalamic noradrenaline content, but not dopamine content, indicating that DSP4 selectively inhibits noradrenergic neurotransmission. DSP4 significantly blocked progesterone-induced increase in serum luteinizing hormone (LH) concentrations as well as GnRH release from hypothalamic fragments incubated in vitro. DSP4 concomitantly down-regulated GnRH mRNA levels in the preoptic area, as determined by competitive reverse transcription-polymerase chain reaction. DSP4 also clearly down-regulated progesterone-induced GnRH receptor mRNA levels in the pituitary, whereas it failed to alter LHβ mRNA levels. In summary, blockade of noradrenergic neurotransmission with DSP4 resulted in profound reductions of hypothalamic GnRH and pituitary GnRH receptor gene expression.

Original languageEnglish
Pages (from-to)911-918
Number of pages8
JournalJournal of Neuroendocrinology
Volume10
Issue number12
DOIs
Publication statusPublished - 1998 Dec 1
Externally publishedYes

Fingerprint

LHRH Receptors
Neurotoxins
Gonadotropin-Releasing Hormone
Steroids
Gene Expression
Progesterone
Synaptic Transmission
Preoptic Area
Luteinizing Hormone
Messenger RNA
Pituitary Hormone-Regulating Hormone Receptors
Pituitary Hormone-Releasing Hormones
Pituitary Gonadotropins
DSP 4
Intraperitoneal Injections
Reverse Transcription
Estradiol
Dopamine
Norepinephrine
Polymerase Chain Reaction

Keywords

  • DSP4
  • GnRH
  • GnRH receptor
  • Hypothalamus
  • Noradrenaline

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Noradrenergic neurotoxin suppresses gonadotropin-releasing hormone (GnRH) and GnRH receptor gene expression in ovariectomized and steroid-treated rats. / Kang, S. S.; Son, Gi Hoon; Seong, Jae Young; Choi, D.; Kwon, H. B.; Lee, C. C.; Kim, K.

In: Journal of Neuroendocrinology, Vol. 10, No. 12, 01.12.1998, p. 911-918.

Research output: Contribution to journalArticle

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