Notch interferes with the scaffold function of JNK-interacting protein 1 to inhibit the JNK signaling pathway

Jin Woo Kim, Myung Jin Kim, Kwang Je Kim, Hee Jae Yun, Ji Soo Chae, Sang Gil Hwang, Tong Shin Chang, Hee Sae Park, Kang Woo Lee, Pyung Lim Han, Ssang Goo Cho, Tae Wan Kim, Eui Ju Choi

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The transmembrane protein Notch is cleaved by γ-secretase to yield an active form. Notch intracellular domain (Notch-IC), in response to the binding of ligands, such as Jagged. Notch-IC contributes to the regulation of a variety of cellular events, including cell fate determination during embryonic development as well as cell growth, differentiation, and survival. We now show that Notch1-IC suppresses the scaffold activity of c-Jun N-terminal kinase (JNK)-interacting protein 1 (JIP1) in the JNK signaling pathway. Notch1-IC physically associated with the JNK binding domain of JIP1 and thereby interfered with the interaction between JIP1 and JNK. JIP1 mediated the activation of JNK1 induced by glucose deprivation in mouse embryonic fibroblasts, and ectopic expression of Notch1-IC inhibited JNK activation and apoptosis triggered by glucose deprivation. Taken together, these findings suggest that Notch1-IC negatively regulates the JNK pathway by disrupting the scaffold function of JIP1.

Original languageEnglish
Pages (from-to)14308-14313
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number40
DOIs
Publication statusPublished - 2005 Oct 4

Fingerprint

JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 8
Proteins
Notch Receptors
Glucose
Amyloid Precursor Protein Secretases
Embryonic Development
Cell Differentiation
Cell Survival
Fibroblasts
Apoptosis
Ligands
Growth

Keywords

  • Apoptosis
  • Glucose deprivation
  • Scaffold

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Notch interferes with the scaffold function of JNK-interacting protein 1 to inhibit the JNK signaling pathway. / Kim, Jin Woo; Kim, Myung Jin; Kim, Kwang Je; Yun, Hee Jae; Chae, Ji Soo; Hwang, Sang Gil; Chang, Tong Shin; Park, Hee Sae; Lee, Kang Woo; Han, Pyung Lim; Cho, Ssang Goo; Kim, Tae Wan; Choi, Eui Ju.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 40, 04.10.2005, p. 14308-14313.

Research output: Contribution to journalArticle

Kim, JW, Kim, MJ, Kim, KJ, Yun, HJ, Chae, JS, Hwang, SG, Chang, TS, Park, HS, Lee, KW, Han, PL, Cho, SG, Kim, TW & Choi, EJ 2005, 'Notch interferes with the scaffold function of JNK-interacting protein 1 to inhibit the JNK signaling pathway', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 40, pp. 14308-14313. https://doi.org/10.1073/pnas.0501600102
Kim, Jin Woo ; Kim, Myung Jin ; Kim, Kwang Je ; Yun, Hee Jae ; Chae, Ji Soo ; Hwang, Sang Gil ; Chang, Tong Shin ; Park, Hee Sae ; Lee, Kang Woo ; Han, Pyung Lim ; Cho, Ssang Goo ; Kim, Tae Wan ; Choi, Eui Ju. / Notch interferes with the scaffold function of JNK-interacting protein 1 to inhibit the JNK signaling pathway. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 40. pp. 14308-14313.
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