Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in abscisic acid-mediated callose deposition in arabidopsis

Min Kyoung You, Hyun Young Shin, Young Jin Kim, Sung Han Ok, Sung Ki Cho, Ji Ung Jeung, Sang-Dong Yoo, Jeong Kook Kim, Jeong Sheop Shin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Screening of the expressed sequence tag library of the wild rice species Oryza minuta revealed an unknown gene that was rapidly and strongly induced in response to attack by a rice fungal pathogen (Magnaporthe oryzae) and an insect (Nilaparvata lugens) and by wounding, abscisic acid (ABA), and methyl jasmonate treatments. Its recombinant protein was identified as a bifunctional nuclease with both RNase and DNase activities in vitro. This gene was designated OmBBD (for O. minuta bifunctional nuclease in basal defense response). Overexpression of OmBBD in an Arabidopsis (Arabidopsis thaliana) model system caused the constitutive expression of the PDF1.2, ABA1, and AtSAC1 genes, which are involved in priming ABA-mediated callose deposition. This activation of defense responses led to an increased resistance against Botrytis cinerea. atbbd1, the knockout mutant of the Arabidopsis ortholog AtBBD1, was susceptible to attack by B. cinerea and had deficient callose deposition. Overexpression of either OmBBD or AtBBD1 in atbbd1 plants complemented the susceptible phenotype of atbbd1 against B. cinerea as well as the deficiency of callose deposition. We suggest that OmBBD and AtBBD1 have a novel regulatory role in ABA-mediated callose deposition.

Original languageEnglish
Pages (from-to)1015-1029
Number of pages15
JournalPlant Physiology
Volume152
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Serratia marcescens nuclease
Oryza minuta
Abscisic Acid
nucleases
callose
Arabidopsis
abscisic acid
Botrytis cinerea
Magnaporthe
Genes
Botrytis
Deoxyribonucleases
Expressed Sequence Tags
Ribonucleases
Magnaporthe oryzae
wild rice
deoxyribonucleases
Nilaparvata lugens
Recombinant Proteins
knockout mutants

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in abscisic acid-mediated callose deposition in arabidopsis. / You, Min Kyoung; Shin, Hyun Young; Kim, Young Jin; Ok, Sung Han; Cho, Sung Ki; Jeung, Ji Ung; Yoo, Sang-Dong; Kim, Jeong Kook; Shin, Jeong Sheop.

In: Plant Physiology, Vol. 152, No. 2, 01.02.2010, p. 1015-1029.

Research output: Contribution to journalArticle

You, Min Kyoung ; Shin, Hyun Young ; Kim, Young Jin ; Ok, Sung Han ; Cho, Sung Ki ; Jeung, Ji Ung ; Yoo, Sang-Dong ; Kim, Jeong Kook ; Shin, Jeong Sheop. / Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in abscisic acid-mediated callose deposition in arabidopsis. In: Plant Physiology. 2010 ; Vol. 152, No. 2. pp. 1015-1029.
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