Novel calcium-binding GTPase (AtCBG) involved in ABA-mediated salt stress signaling in Arabidopsis

Kandakumar Jayasekaran, Kyung Nam Kim, Munusamy Vivekanandan, Jeong Sheop Shin, Sung Han Ok

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have identified a novel Ca2+-signal sensing GTPase (643 amino acid residues with an estimated molecular mass of 79 kDa) from the Arabidopsis genome database. This protein contains a RHO-like GTPase domain at the N-terminus (15-184 amino acids) and two calcium-binding EF-hand motifs (199-227 and 319-347 amino acids, respectively). It has the capability to bind calcium and hydrolyze GTP; in addition, its GTPase activity is regulated by changes in Ca2+ concentration. The expression of this gene was induced by ABA and salt stresses, and specific knock-out mutants were highly sensitive to ABA and salt treatments. These findings suggest that this protein is a novel ABA- and salt stress-related Ca2+ signal transducer.

Original languageEnglish
Pages (from-to)1255-1262
Number of pages8
JournalPlant Cell Reports
Volume25
Issue number11
DOIs
Publication statusPublished - 2006 Nov 1

Fingerprint

guanosinetriphosphatase
salt stress
Arabidopsis
calcium
amino acids
EF hand motifs
knockout mutants
proteins
molecular weight
salts
gene expression
genome

Keywords

  • Abiotic stress
  • Ca
  • GTP-binding
  • Regulation of gene expression
  • Signal transduction

ASJC Scopus subject areas

  • Plant Science

Cite this

Novel calcium-binding GTPase (AtCBG) involved in ABA-mediated salt stress signaling in Arabidopsis. / Jayasekaran, Kandakumar; Kim, Kyung Nam; Vivekanandan, Munusamy; Shin, Jeong Sheop; Ok, Sung Han.

In: Plant Cell Reports, Vol. 25, No. 11, 01.11.2006, p. 1255-1262.

Research output: Contribution to journalArticle

Jayasekaran, Kandakumar ; Kim, Kyung Nam ; Vivekanandan, Munusamy ; Shin, Jeong Sheop ; Ok, Sung Han. / Novel calcium-binding GTPase (AtCBG) involved in ABA-mediated salt stress signaling in Arabidopsis. In: Plant Cell Reports. 2006 ; Vol. 25, No. 11. pp. 1255-1262.
@article{1683a438099e458ba510d8836f1804bf,
title = "Novel calcium-binding GTPase (AtCBG) involved in ABA-mediated salt stress signaling in Arabidopsis",
abstract = "We have identified a novel Ca2+-signal sensing GTPase (643 amino acid residues with an estimated molecular mass of 79 kDa) from the Arabidopsis genome database. This protein contains a RHO-like GTPase domain at the N-terminus (15-184 amino acids) and two calcium-binding EF-hand motifs (199-227 and 319-347 amino acids, respectively). It has the capability to bind calcium and hydrolyze GTP; in addition, its GTPase activity is regulated by changes in Ca2+ concentration. The expression of this gene was induced by ABA and salt stresses, and specific knock-out mutants were highly sensitive to ABA and salt treatments. These findings suggest that this protein is a novel ABA- and salt stress-related Ca2+ signal transducer.",
keywords = "Abiotic stress, Ca, GTP-binding, Regulation of gene expression, Signal transduction",
author = "Kandakumar Jayasekaran and Kim, {Kyung Nam} and Munusamy Vivekanandan and Shin, {Jeong Sheop} and Ok, {Sung Han}",
year = "2006",
month = "11",
day = "1",
doi = "10.1007/s00299-006-0195-5",
language = "English",
volume = "25",
pages = "1255--1262",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "11",

}

TY - JOUR

T1 - Novel calcium-binding GTPase (AtCBG) involved in ABA-mediated salt stress signaling in Arabidopsis

AU - Jayasekaran, Kandakumar

AU - Kim, Kyung Nam

AU - Vivekanandan, Munusamy

AU - Shin, Jeong Sheop

AU - Ok, Sung Han

PY - 2006/11/1

Y1 - 2006/11/1

N2 - We have identified a novel Ca2+-signal sensing GTPase (643 amino acid residues with an estimated molecular mass of 79 kDa) from the Arabidopsis genome database. This protein contains a RHO-like GTPase domain at the N-terminus (15-184 amino acids) and two calcium-binding EF-hand motifs (199-227 and 319-347 amino acids, respectively). It has the capability to bind calcium and hydrolyze GTP; in addition, its GTPase activity is regulated by changes in Ca2+ concentration. The expression of this gene was induced by ABA and salt stresses, and specific knock-out mutants were highly sensitive to ABA and salt treatments. These findings suggest that this protein is a novel ABA- and salt stress-related Ca2+ signal transducer.

AB - We have identified a novel Ca2+-signal sensing GTPase (643 amino acid residues with an estimated molecular mass of 79 kDa) from the Arabidopsis genome database. This protein contains a RHO-like GTPase domain at the N-terminus (15-184 amino acids) and two calcium-binding EF-hand motifs (199-227 and 319-347 amino acids, respectively). It has the capability to bind calcium and hydrolyze GTP; in addition, its GTPase activity is regulated by changes in Ca2+ concentration. The expression of this gene was induced by ABA and salt stresses, and specific knock-out mutants were highly sensitive to ABA and salt treatments. These findings suggest that this protein is a novel ABA- and salt stress-related Ca2+ signal transducer.

KW - Abiotic stress

KW - Ca

KW - GTP-binding

KW - Regulation of gene expression

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=33750302047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750302047&partnerID=8YFLogxK

U2 - 10.1007/s00299-006-0195-5

DO - 10.1007/s00299-006-0195-5

M3 - Article

C2 - 16832621

AN - SCOPUS:33750302047

VL - 25

SP - 1255

EP - 1262

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 11

ER -