Novel Cyanostilbene-Based Fluorescent Chemoprobe for Hydroxyl Radicals and Its Two-Photon Bioimaging in Living Cells

Na Young Lim, Junho Ahn, Miae Won, Wonjin Choi, Jong Seung Kim, Jong Hwa Jung

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A novel cyanostilbene derivative as a selective fluorescent chemoprobe for hydroxyl radicals was synthesized. The chemoprobe shows strong green emission in aqueous solution with the addition of hydroxyl radicals. Conversely, negligible emission changes are observed upon addition of other reactive oxygen species. The chemoprobe 1 shows high sensitivity, having the low detection limit of ∼1.0 × 10-7 M. Furthermore, the fluorescent chemoprobe exhibits low cytotoxicity and is effectively applied to bioimaging of hydroxyl radicals by two-photon confocal microscopy in HeLa cells. These results indicate that the new chemoprobe has great potential for bioimaging in vivo and in vitro systems.

Original languageEnglish
Pages (from-to)936-942
Number of pages7
JournalACS Applied Bio Materials
Volume2
Issue number2
DOIs
Publication statusPublished - 2019 Feb 18

Keywords

  • cyanostilbene
  • fluorescent chemoprobe
  • hydroxyl radical
  • living cell
  • reactive oxygen species

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

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