Novel Detection Method for Circulating EGFR Tumor DNA Using Gravitationally Condensed Gold Nanoparticles and Catalytic Walker DNA

Juneseok You, Chanho Park, Kuewhan Jang, Jinsung Park, Sungsoo Na

Research output: Contribution to journalArticlepeer-review

Abstract

The detection of circulating tumor DNA is a major challenge in liquid biopsies for cancer. Conventionally, quantitative polymerase chain reactions or next-generation sequencing are used to detect circulating tumor DNA; however, these techniques require significant expertise, and are expensive. Owing to the increasing demand for a simple diagnostic method and constant monitoring of cancer, a cost-effective detection technique that can be conducted by non-experts is required. The aim of this study was to detect the circulating tumor DNA containing the epidermal growth factor receptor (EGFR) exon 19 deletion, which frequently occurs in lung cancer. By applying walker DNA to a catalytic hairpin assembly and using the differential dispersibility of gold nanoparticles, we detected EGFR exon 19 deletion mutant #2 DNA associated with lung cancer. Our sensing platform exhibited a limit of detection of 38.5 aM and a selectivity of 0.1% for EGFR exon 19 wild-type DNA. Moreover, we tested and compared EGFR exon 19 deletion mutants #1 and #3 to evaluate the effect of base pair mismatches on the performance of the said technique.

Original languageEnglish
Article number3301
JournalMaterials
Volume15
Issue number9
DOIs
Publication statusPublished - 2022 May 1

Keywords

  • circulating tumor DNA
  • gold nanoparticle
  • UV-vis absorbance
  • walker DNA

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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