@article{ef434bf9b4a04c228f5099f1c8d22c1b,
title = "Novel Detection Method for Circulating EGFR Tumor DNA Using Gravitationally Condensed Gold Nanoparticles and Catalytic Walker DNA",
abstract = "The detection of circulating tumor DNA is a major challenge in liquid biopsies for cancer. Conventionally, quantitative polymerase chain reactions or next-generation sequencing are used to detect circulating tumor DNA; however, these techniques require significant expertise, and are expensive. Owing to the increasing demand for a simple diagnostic method and constant monitoring of cancer, a cost-effective detection technique that can be conducted by non-experts is required. The aim of this study was to detect the circulating tumor DNA containing the epidermal growth factor receptor (EGFR) exon 19 deletion, which frequently occurs in lung cancer. By applying walker DNA to a catalytic hairpin assembly and using the differential dispersibility of gold nanoparticles, we detected EGFR exon 19 deletion mutant #2 DNA associated with lung cancer. Our sensing platform exhibited a limit of detection of 38.5 aM and a selectivity of 0.1% for EGFR exon 19 wild-type DNA. Moreover, we tested and compared EGFR exon 19 deletion mutants #1 and #3 to evaluate the effect of base pair mismatches on the performance of the said technique.",
keywords = "UV-vis absorbance, circulating tumor DNA, gold nanoparticle, walker DNA",
author = "Juneseok You and Chanho Park and Kuewhan Jang and Jinsung Park and Sungsoo Na",
note = "Funding Information: Supplementary Materials: The following are available online at www.mdpi.com/xxx/s1, Figure S1: 10.3390/ma15093301/s1, Figure S1: Schematic image of EGFR exon 19 deletion types with codon Schematic image of EGFR exon 19 deletion types with codon name and number, Figure S2: Detailed name and number, Figure S2: Detailed electrophoresis band line explanation, Figure S3: 800 mM of electrophoresis band line explanation, Figure S3: 800 mM of NaCl precipitation test, Figure S4: 96-NaCl precipitation test, Figure S4: 96-well plate picture of detection of EGFR exon 19 mutation #2 well plate picture of detection of EGFR exon 19 mutation #2 DNA., Figure S5: UV absorbance spec-DNA, Figure S5: UV absorbance spectrum plot of detection of EGFR exon 19 mutation #2, Figure S6: trum plot of detection of EGFR exon 19 mutation #2, Figure S6: Agarose gel electrophoresis test of Agarose gel electrophoresis test of selective activation of walker DNA, Table S1: Literature studies of detection of EGFR DNA. Author Contributions: J.Y., C.P. conceived and designed the experiments and wrote the manuscript; J.Y. performed all experiments and wrote the manuscript; J.Y. and K.J. analyzed the UV-vis absorbance data. J.Y. carried out electrophoresis analysis. J.P. and S.N. supervised all the experiments, analysis, and writing the manuscripts. All authors have read and agreed to the published analysis, and writing the manuscripts. All authors have read and agreed to the published version of the manuscript. Funding: This work was supported by the National Research Foundation of Korea (NRF) under Grant No. NRF-2016R1A5A1010148, NRF-2020R1F1A1075581, and which is funded by the Ministry of Science, ICT and Future Planning. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = may,
day = "1",
doi = "10.3390/ma15093301",
language = "English",
volume = "15",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "9",
}
