Novel genes and hormonal regulation for gonadal development during embryogenesis in chickens

Whasun Lim, Gwonhwa Song

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Asymmetrical gonadal morphogenesis is well known in female chickens in contrast to males where both gonads develop symmetrically. However, only a few genes have been reported to determine differential morphology between female and male gonads in chicken and their mechanisms of action are unclear. Therefore, we focused on three genes (TOM1L1, TTR, and ZEB1) that are related to cellular proliferation and embryonic development based on previous study indicating up- or down-regulated transcripts in the asymmetric female gonads between embryonic day 6 (E6) and E9 by microarray analyses. To define the validity of the gene expression pattern discovered, q-PCR and in situ hybridization analyses were performed. In the left female gonad between E6 and E9 the expression of TOM1L1, TTR and ZEB1 increased at E9. On the other hand, TOM1L1 and TTR increased significantly in both male gonads between E6 and E9. In addition, recombinant FSH and LH stimulated proliferation of gonadal cells and influenced expression of selected genes in chickens. This suggests that hormonal regulation is involved in growth and development in the embryonic gonad of chickens. Collectively, the results show differential gene expression between the left and right gonads in chicken embryos and that of is regulated by gonadotropin. These results provide novel insights into candidate genes regulating gonad development and differentiation.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume211
DOIs
Publication statusPublished - 2015 Jan 5

Fingerprint

Gonads
Embryonic Development
Chickens
Genes
Gene Expression
Cell Proliferation
Microarray Analysis
Gonadotropins
Morphogenesis
Growth and Development
In Situ Hybridization
Embryonic Structures
Polymerase Chain Reaction

Keywords

  • Asymmetric development
  • Chicken
  • Embryo
  • Gonad
  • Reproductive hormones

ASJC Scopus subject areas

  • Endocrinology

Cite this

Novel genes and hormonal regulation for gonadal development during embryogenesis in chickens. / Lim, Whasun; Song, Gwonhwa.

In: General and Comparative Endocrinology, Vol. 211, 05.01.2015, p. 20-27.

Research output: Contribution to journalArticle

@article{62d54a0ec2f34994a655357bcfc6a149,
title = "Novel genes and hormonal regulation for gonadal development during embryogenesis in chickens",
abstract = "Asymmetrical gonadal morphogenesis is well known in female chickens in contrast to males where both gonads develop symmetrically. However, only a few genes have been reported to determine differential morphology between female and male gonads in chicken and their mechanisms of action are unclear. Therefore, we focused on three genes (TOM1L1, TTR, and ZEB1) that are related to cellular proliferation and embryonic development based on previous study indicating up- or down-regulated transcripts in the asymmetric female gonads between embryonic day 6 (E6) and E9 by microarray analyses. To define the validity of the gene expression pattern discovered, q-PCR and in situ hybridization analyses were performed. In the left female gonad between E6 and E9 the expression of TOM1L1, TTR and ZEB1 increased at E9. On the other hand, TOM1L1 and TTR increased significantly in both male gonads between E6 and E9. In addition, recombinant FSH and LH stimulated proliferation of gonadal cells and influenced expression of selected genes in chickens. This suggests that hormonal regulation is involved in growth and development in the embryonic gonad of chickens. Collectively, the results show differential gene expression between the left and right gonads in chicken embryos and that of is regulated by gonadotropin. These results provide novel insights into candidate genes regulating gonad development and differentiation.",
keywords = "Asymmetric development, Chicken, Embryo, Gonad, Reproductive hormones",
author = "Whasun Lim and Gwonhwa Song",
year = "2015",
month = "1",
day = "5",
doi = "10.1016/j.ygcen.2014.11.009",
language = "English",
volume = "211",
pages = "20--27",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Novel genes and hormonal regulation for gonadal development during embryogenesis in chickens

AU - Lim, Whasun

AU - Song, Gwonhwa

PY - 2015/1/5

Y1 - 2015/1/5

N2 - Asymmetrical gonadal morphogenesis is well known in female chickens in contrast to males where both gonads develop symmetrically. However, only a few genes have been reported to determine differential morphology between female and male gonads in chicken and their mechanisms of action are unclear. Therefore, we focused on three genes (TOM1L1, TTR, and ZEB1) that are related to cellular proliferation and embryonic development based on previous study indicating up- or down-regulated transcripts in the asymmetric female gonads between embryonic day 6 (E6) and E9 by microarray analyses. To define the validity of the gene expression pattern discovered, q-PCR and in situ hybridization analyses were performed. In the left female gonad between E6 and E9 the expression of TOM1L1, TTR and ZEB1 increased at E9. On the other hand, TOM1L1 and TTR increased significantly in both male gonads between E6 and E9. In addition, recombinant FSH and LH stimulated proliferation of gonadal cells and influenced expression of selected genes in chickens. This suggests that hormonal regulation is involved in growth and development in the embryonic gonad of chickens. Collectively, the results show differential gene expression between the left and right gonads in chicken embryos and that of is regulated by gonadotropin. These results provide novel insights into candidate genes regulating gonad development and differentiation.

AB - Asymmetrical gonadal morphogenesis is well known in female chickens in contrast to males where both gonads develop symmetrically. However, only a few genes have been reported to determine differential morphology between female and male gonads in chicken and their mechanisms of action are unclear. Therefore, we focused on three genes (TOM1L1, TTR, and ZEB1) that are related to cellular proliferation and embryonic development based on previous study indicating up- or down-regulated transcripts in the asymmetric female gonads between embryonic day 6 (E6) and E9 by microarray analyses. To define the validity of the gene expression pattern discovered, q-PCR and in situ hybridization analyses were performed. In the left female gonad between E6 and E9 the expression of TOM1L1, TTR and ZEB1 increased at E9. On the other hand, TOM1L1 and TTR increased significantly in both male gonads between E6 and E9. In addition, recombinant FSH and LH stimulated proliferation of gonadal cells and influenced expression of selected genes in chickens. This suggests that hormonal regulation is involved in growth and development in the embryonic gonad of chickens. Collectively, the results show differential gene expression between the left and right gonads in chicken embryos and that of is regulated by gonadotropin. These results provide novel insights into candidate genes regulating gonad development and differentiation.

KW - Asymmetric development

KW - Chicken

KW - Embryo

KW - Gonad

KW - Reproductive hormones

UR - http://www.scopus.com/inward/record.url?scp=84922748947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922748947&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2014.11.009

DO - 10.1016/j.ygcen.2014.11.009

M3 - Article

C2 - 25452029

AN - SCOPUS:84922748947

VL - 211

SP - 20

EP - 27

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

ER -