Abstract
MicroRNAs (miRNAs) play pivotal roles in tumorigenesis as either tumor suppressors or oncogenes. In the present study, we discovered and demonstrated the tumor suppressive function of a novel miRNA miR-5582-5p. miR-5582-5p induced apoptosis and cell cycle arrest in cancer cells, but not in normal cells. GAB1, SHC1, and CDK2 were identified as direct targets of miR-5582-5p. Knockdown of GAB1/SHC1 or CDK2 phenocopied the apoptotic or cell cycle arrest-inducing function of miR-5582-5p, respectively. The expression of miR-5582-5p was lower in tumor tissues than in adjacent normal tissues of colorectal cancer patients, while the expression of the target proteins exhibited patterns opposite to that of miR-5582-5p. Intratumoral injection of a miR-5582-5p mimic or induced expression of miR-5582-5p in tumor cells suppressed tumor growth in HCT116 xenografts. Collectively, our results suggest a novel tumor suppressive function for miR-5582-5p and its potential applicability for tumor control.
Original language | English |
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Pages (from-to) | 1926-1937 |
Number of pages | 12 |
Journal | Biochimica et Biophysica Acta - Molecular Basis of Disease |
Volume | 1862 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2016 Oct 1 |
Keywords
- CDK2
- GAB1
- MicroRNA
- SHC1
- Tumor suppressor
- miR-5582-5p
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology