Novel PCR-restriction fragment length polymorphism analysis for rapid typing of Staphylococcal cassette chromosome mec elements

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14 Citations (Scopus)

Abstract

We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.

Original languageEnglish
Pages (from-to)236-238
Number of pages3
JournalJournal of Clinical Microbiology
Volume44
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

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Restriction Fragment Length Polymorphisms
Chromosomes
Polymerase Chain Reaction
Methicillin-Resistant Staphylococcus aureus
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

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abstract = "We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.",
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