Novel protein kinase interacts with the Cucumber mosaic virus 1a methyltransferase domain

Min Jung Kim, Byung Kook Ham, Kyung Hee Paek

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Cucumber mosaic virus (CMV)-encoded 1a protein has been implicated to play a role in replication of the viral genome along with 2a and one or more host factors. To identify the host cell factors interacting with CMV 1a, we used the yeast two-hybrid system using tobacco cDNA library. One of the cDNA clones encoded a protein homologous to the Arabidopsis putative protein kinase and was designated as Tcoi2 (Tobacco CMV 1a interacting protein 2). Tcoi2 specifically interacted with methyltransferase (MT) domain of CMV 1a protein in yeast cell. In vitro analyses using recombinant proteins showed that Tcoi2 also specifically interacted with CMV 1a MT domain. Tcoi2 did not have autophosphorylation activity but phosphorylated CMV 1a MT domain. Analysis of the subcellular localization of the Tcoi2 fused to GFP demonstrated that it is targeted to the endoplasmic reticulum. These results suggest Tcoi2 as a novel host factor that is capable of interacting and phosphorylating MT domain of CMV 1a protein.

Original languageEnglish
Pages (from-to)228-235
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume340
Issue number1
DOIs
Publication statusPublished - 2006 Feb 3

Fingerprint

Cucumovirus
Methyltransferases
Viruses
Protein Kinases
Tobacco
Yeast
Arabidopsis Proteins
Tobacco Mosaic Virus
Two-Hybrid System Techniques
Viral Genome
Gene Library
Recombinant Proteins
Proteins
Endoplasmic Reticulum
Hybrid systems
Complementary DNA
Clone Cells
Yeasts
Genes
Cells

Keywords

  • CMV 1a protein
  • Kinase
  • Replication complex
  • Tcoi2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Novel protein kinase interacts with the Cucumber mosaic virus 1a methyltransferase domain. / Kim, Min Jung; Ham, Byung Kook; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 340, No. 1, 03.02.2006, p. 228-235.

Research output: Contribution to journalArticle

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