Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation

Sung Tae Kim, Dong Joo Kim, Tae Jin Kim, Deok Won Seo, Tae Hong Kim, Seung Yong Lee, Kwanghee Kim, Kyung-Mi Lee, Sang Kwon Lee

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4+ T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4+ T lymphocytes. Fabricated SiNW arrays easily separated the CD4+ T lymphocytes from the mouse whole splenocytes with over ∼88% purity and demonstrated tight attachment to CD4+ T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.

Original languageEnglish
Pages (from-to)2877-2883
Number of pages7
JournalNano Letters
Volume10
Issue number8
DOIs
Publication statusPublished - 2010 Aug 11

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Keywords

  • CD4 T lymphocyte
  • cell separation
  • Silicon nanowire
  • streptavidin

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Kim, S. T., Kim, D. J., Kim, T. J., Seo, D. W., Kim, T. H., Lee, S. Y., Kim, K., Lee, K-M., & Lee, S. K. (2010). Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation. Nano Letters, 10(8), 2877-2883. https://doi.org/10.1021/nl100942p