Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation

Sung Tae Kim, Dong Joo Kim, Tae Jin Kim, Deok Won Seo, Tae Hong Kim, Seung Yong Lee, Kwanghee Kim, Kyung-Mi Lee, Sang Kwon Lee

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4+ T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4+ T lymphocytes. Fabricated SiNW arrays easily separated the CD4+ T lymphocytes from the mouse whole splenocytes with over ∼88% purity and demonstrated tight attachment to CD4+ T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.

Original languageEnglish
Pages (from-to)2877-2883
Number of pages7
JournalNano Letters
Volume10
Issue number8
DOIs
Publication statusPublished - 2010 Aug 11

Fingerprint

Streptavidin
T-cells
lymphocytes
Silicon
Nanowires
nanowires
silicon
biotin
Biotin
attachment
mice
affinity
Nanostructures
purity
Cells
Scanning electron microscopy
scanning electron microscopy
cells

Keywords

  • CD4 T lymphocyte
  • cell separation
  • Silicon nanowire
  • streptavidin

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Kim, S. T., Kim, D. J., Kim, T. J., Seo, D. W., Kim, T. H., Lee, S. Y., ... Lee, S. K. (2010). Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation. Nano Letters, 10(8), 2877-2883. https://doi.org/10.1021/nl100942p

Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation. / Kim, Sung Tae; Kim, Dong Joo; Kim, Tae Jin; Seo, Deok Won; Kim, Tae Hong; Lee, Seung Yong; Kim, Kwanghee; Lee, Kyung-Mi; Lee, Sang Kwon.

In: Nano Letters, Vol. 10, No. 8, 11.08.2010, p. 2877-2883.

Research output: Contribution to journalArticle

Kim, ST, Kim, DJ, Kim, TJ, Seo, DW, Kim, TH, Lee, SY, Kim, K, Lee, K-M & Lee, SK 2010, 'Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation', Nano Letters, vol. 10, no. 8, pp. 2877-2883. https://doi.org/10.1021/nl100942p
Kim, Sung Tae ; Kim, Dong Joo ; Kim, Tae Jin ; Seo, Deok Won ; Kim, Tae Hong ; Lee, Seung Yong ; Kim, Kwanghee ; Lee, Kyung-Mi ; Lee, Sang Kwon. / Novel streptavidin-functionalized silicon nanowire arrays for CD4 + T lymphocyte separation. In: Nano Letters. 2010 ; Vol. 10, No. 8. pp. 2877-2883.
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