Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate

Hyun Jung Lee, Hyun Sun Lee, Jang Won Choi, Kyung Soo Ra, Jin Man Kim, Hyung Joo Suh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC 50 = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC 50) were 2.7 and 1.5 mg/mL, respectively.

Original languageEnglish
Pages (from-to)11522-11525
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number21
DOIs
Publication statusPublished - 2011 Nov 9

Fingerprint

Glucosidases
tripeptides
glucosidases
Silk
cocoons
silk
hydrolysates
peptides
Peptides
glycyl-tyrosyl-glycine
inhibitory concentration 50
Gels
High Pressure Liquid Chromatography
gels
hypoglycemic agents
Ultrafiltration
ultrafiltration
Hypoglycemic Agents
Gel Chromatography
Amino Acid Sequence

Keywords

  • α-glucosidase
  • cocoon
  • diabetes
  • silk peptide E5K6

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate. / Lee, Hyun Jung; Lee, Hyun Sun; Choi, Jang Won; Ra, Kyung Soo; Kim, Jin Man; Suh, Hyung Joo.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 21, 09.11.2011, p. 11522-11525.

Research output: Contribution to journalArticle

Lee, Hyun Jung ; Lee, Hyun Sun ; Choi, Jang Won ; Ra, Kyung Soo ; Kim, Jin Man ; Suh, Hyung Joo. / Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 21. pp. 11522-11525.
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AU - Kim, Jin Man

AU - Suh, Hyung Joo

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