Novosphingobium aureum sp. Nov., a marine bacterium isolated from salt flat sediment

A Gram-stain-negative, aerobic, pale yellow-coloured, rod-shaped marine bacterium designated strain YJ-S2-02^T was isolated from salt flat sediment sampled in Yongyu-do, Republic of Korea. Strain YJ-S2-02^T grew at pH 6.0–9.0 (optimum, pH 7.0), 10–40 °C (optimum, 30 °C) and with optimum 1% (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain YJ-S2-02^T was closely related to Novosphingobium naphthalenivorans NBRC 102051^T (97.8%) followed by Novosphingobium mathurense SM117^T (97.5%), Novosphingobium indicum H25^T (97.3%), Novosphingobium pentaromativorans US6-1^T (96.8%), Novosphingobium fontis STM-14^T (96.6%), Novosphingobium endophyticum EGI60015^T (96.5%), Novosphingobium naphthae D39^T (96.5%) and Novo-sphingobium malaysiense MUSC 273^T (95.9%). The average nucleotide identity and estimated DNA–DNA hybridization values between YJ-S2-02^T and related type strains were 77.0–77.9% and 19.1–24.0%. Strain YJ-S2-02^T was characterized as having Q-10 as the predominant respiratory quinone and the principal fatty acids (>10%) were summed feature 8 (C_18:1 ω6c/ω7c, 20.7%), C_18:3 ω6c (16.3%) and C_17:1 ω6c (11.8%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingolipids and two unidentified lipids. The DNA G+C content of strain YJ-S2-02^T was 65.6 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S2-02^T should be classified as representing a novel species within the genus Novosphingobium, for which name Novosphingobium aureum is proposed, with the type strain YJ-S2-02^T (=KACC 21677^T =KCTC 72891^T=JCM 33996^T).