NPS2143 Inhibits MUC5AC and Proinflammatory Mediators in Cigarette Smoke Extract (CSE)-Stimulated Human Airway Epithelial Cells

Jae Won Lee, Ji Won Park, Ok Kyoung Kwon, Hee Jae Lee, Hye Gwang Jeong, Jae-Hong Kim, Sei Ryang Oh, Kyoung Seop Ahn

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Mucus overproduction is a fundamental hallmark of COPD that is caused by exposure to cigarette smoke. MUC5AC is one of the main mucin genes expressed in the respiratory epithelium, and its transcriptional upregulation often correlates with increased mucus secretion. Calcium-sensing receptor (CaSR) antagonists have been reported to possess anti-inflammatory effects. The purpose of the present study was to investigate the protective role of NPS2143, a selective CaSR antagonist on cigarette smoke extract (CSE)-stimulated NCI-H292 mucoepidermoid human lung cells. Treatment of NPS2143 significantly inhibited the expression of MUC5AC in CSE-stimulated H292 cells. NPS2143 reduced the expression of MMP-9 in CSE-stimulated H292 cells. NPS2143 also decreased the release of proinflammatory cytokines such as IL-6 and TNF-α in CSE-stimulated H292 cells. Furthermore, NPS2143 attenuated the activation of MAPKs (JNK, p38, and ERK) and inhibited the nuclear translocation of NF-κB in CSE-stimulated H292 cells. These results indicate that NPS2143 had a therapeutic potential in COPD.

Original languageEnglish
Pages (from-to)184-194
Number of pages11
JournalInflammation
Volume40
Issue number1
DOIs
Publication statusPublished - 2017 Feb 1

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Keywords

  • chronic obstructive pulmonary disease
  • cigarette smoke
  • MAPKs
  • MUC5AC
  • NF-κB
  • NPS2143

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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