Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology

Jeong Ki Kim, Arghavan Louhghalam, Geonhui Lee, Benjamin W. Schafer, Denis Wirtz, Dong-Hwee Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The distinct spatial architecture of the apical actin cables (or actin cap) facilitates rapid biophysical signaling between extracellular mechanical stimuli and intracellular responses, including nuclear shaping, cytoskeletal remodeling, and the mechanotransduction of external forces into biochemical signals. These functions are abrogated in lamin A/C-deficient mouse embryonic fibroblasts that recapitulate the defective nuclear organization of laminopathies, featuring disruption of the actin cap. However, how nuclear lamin A/C mediates the ability of the actin cap to regulate nuclear morphology remains unclear. Here, we show that lamin A/C expressing cells can form an actin cap to resist nuclear deformation in response to physiological mechanical stresses. This study reveals how the nuclear lamin A/C-mediated formation of the perinuclear apical actin cables protects the nuclear structural integrity from extracellular physical disturbances. Our findings highlight the role of the physical interactions between the cytoskeletal network and the nucleus in cellular mechanical homeostasis.

Original languageEnglish
Article number2123
JournalNature communications
Volume8
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Lamin Type A
harnesses
caps
cables
Actins
Cables
nuclear deformation
homeostasis
fibroblasts
stimuli
integrity
mice
Mechanical Stress
disturbances
Structural integrity
Fibroblasts
nuclei
Homeostasis
cells
interactions

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology. / Kim, Jeong Ki; Louhghalam, Arghavan; Lee, Geonhui; Schafer, Benjamin W.; Wirtz, Denis; Kim, Dong-Hwee.

In: Nature communications, Vol. 8, No. 1, 2123, 01.12.2017.

Research output: Contribution to journalArticle

Kim, Jeong Ki ; Louhghalam, Arghavan ; Lee, Geonhui ; Schafer, Benjamin W. ; Wirtz, Denis ; Kim, Dong-Hwee. / Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology. In: Nature communications. 2017 ; Vol. 8, No. 1.
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