Abstract
The distinct spatial architecture of the apical actin cables (or actin cap) facilitates rapid biophysical signaling between extracellular mechanical stimuli and intracellular responses, including nuclear shaping, cytoskeletal remodeling, and the mechanotransduction of external forces into biochemical signals. These functions are abrogated in lamin A/C-deficient mouse embryonic fibroblasts that recapitulate the defective nuclear organization of laminopathies, featuring disruption of the actin cap. However, how nuclear lamin A/C mediates the ability of the actin cap to regulate nuclear morphology remains unclear. Here, we show that lamin A/C expressing cells can form an actin cap to resist nuclear deformation in response to physiological mechanical stresses. This study reveals how the nuclear lamin A/C-mediated formation of the perinuclear apical actin cables protects the nuclear structural integrity from extracellular physical disturbances. Our findings highlight the role of the physical interactions between the cytoskeletal network and the nucleus in cellular mechanical homeostasis.
| Original language | English |
|---|---|
| Article number | 2123 |
| Journal | Nature communications |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2017 Dec 1 |
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ASJC Scopus subject areas
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)
Cite this
Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology. / Kim, Jeong Ki; Louhghalam, Arghavan; Lee, Geonhui; Schafer, Benjamin W.; Wirtz, Denis; Kim, Dong-Hwee.
In: Nature communications, Vol. 8, No. 1, 2123, 01.12.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology
AU - Kim, Jeong Ki
AU - Louhghalam, Arghavan
AU - Lee, Geonhui
AU - Schafer, Benjamin W.
AU - Wirtz, Denis
AU - Kim, Dong-Hwee
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The distinct spatial architecture of the apical actin cables (or actin cap) facilitates rapid biophysical signaling between extracellular mechanical stimuli and intracellular responses, including nuclear shaping, cytoskeletal remodeling, and the mechanotransduction of external forces into biochemical signals. These functions are abrogated in lamin A/C-deficient mouse embryonic fibroblasts that recapitulate the defective nuclear organization of laminopathies, featuring disruption of the actin cap. However, how nuclear lamin A/C mediates the ability of the actin cap to regulate nuclear morphology remains unclear. Here, we show that lamin A/C expressing cells can form an actin cap to resist nuclear deformation in response to physiological mechanical stresses. This study reveals how the nuclear lamin A/C-mediated formation of the perinuclear apical actin cables protects the nuclear structural integrity from extracellular physical disturbances. Our findings highlight the role of the physical interactions between the cytoskeletal network and the nucleus in cellular mechanical homeostasis.
AB - The distinct spatial architecture of the apical actin cables (or actin cap) facilitates rapid biophysical signaling between extracellular mechanical stimuli and intracellular responses, including nuclear shaping, cytoskeletal remodeling, and the mechanotransduction of external forces into biochemical signals. These functions are abrogated in lamin A/C-deficient mouse embryonic fibroblasts that recapitulate the defective nuclear organization of laminopathies, featuring disruption of the actin cap. However, how nuclear lamin A/C mediates the ability of the actin cap to regulate nuclear morphology remains unclear. Here, we show that lamin A/C expressing cells can form an actin cap to resist nuclear deformation in response to physiological mechanical stresses. This study reveals how the nuclear lamin A/C-mediated formation of the perinuclear apical actin cables protects the nuclear structural integrity from extracellular physical disturbances. Our findings highlight the role of the physical interactions between the cytoskeletal network and the nucleus in cellular mechanical homeostasis.
UR - http://www.scopus.com/inward/record.url?scp=85038827849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85038827849&partnerID=8YFLogxK
U2 - 10.1038/s41467-017-02217-5
DO - 10.1038/s41467-017-02217-5
M3 - Article
C2 - 29242553
AN - SCOPUS:85038827849
VL - 8
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 2123
ER -