Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis

Young-Gyu Ko, Young Sun Kang, Eun Kyoung Kim, Sang Gyu Park, Sunghoon Kim

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Human aminoacyl-tRNA synthetases (ARSs) are normally located in cytoplasm and are involved in protein synthesis. In the present work, we found that human methionyl-tRNA synthetase (MRS) was translocated to nucleolus in proliferative cells, but disappeared in quiescent cells. The nucleolar localization of MRS was triggered by various growth factors such as insulin, PDGF, and EGF. The presence of MRS in nucleoli depended on the integrity of RNA and the activity of RNA polymerase I in the nucleolus. The ribosomal RNA synthesis was specifically decreased by the treatment of anti- MRS antibody as determined by nuclear run-on assay and immunostaining with anti-Br antibody after incorporating Br-UTP into nascent RNA. Thus, human MRS plays a role in the biogenesis of rRNA in nucleoli, while it is catalytically involved in protein synthesis in cytoplasm.

Original languageEnglish
Pages (from-to)567-574
Number of pages8
JournalJournal of Cell Biology
Volume149
Issue number3
DOIs
Publication statusPublished - 2000 May 1
Externally publishedYes

Fingerprint

Methionine-tRNA Ligase
Ribosomal RNA
Cytoplasm
RNA
RNA Polymerase I
Amino Acyl-tRNA Synthetases
Uridine Triphosphate
Epidermal Growth Factor
Anti-Idiotypic Antibodies
Intercellular Signaling Peptides and Proteins
Proteins
Insulin
Antibodies

Keywords

  • Growth signal
  • Methionyl-tRNA synthetase
  • Nucleoli
  • Ribosomal RNA synthesis
  • RNA polymerase I

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis. / Ko, Young-Gyu; Kang, Young Sun; Kim, Eun Kyoung; Park, Sang Gyu; Kim, Sunghoon.

In: Journal of Cell Biology, Vol. 149, No. 3, 01.05.2000, p. 567-574.

Research output: Contribution to journalArticle

Ko, Young-Gyu ; Kang, Young Sun ; Kim, Eun Kyoung ; Park, Sang Gyu ; Kim, Sunghoon. / Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis. In: Journal of Cell Biology. 2000 ; Vol. 149, No. 3. pp. 567-574.
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