Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii: Overexpression, crystallization and preliminary X-ray crystallographic analysis

K. Min, Hyun Kyu Song, C. Chang, Young Lee Jae Young Lee, Hyun Eom Soo Hyun Eom, Kyu Kim Kyeong Kyu Kim, Gyu Yu Yeon Gyu Yu, Won Suh Se Won Suh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nucleoside diphosphate (NDP) kinase is a key enzyme in maintaining cellular pools of all nucleoside triphosphates. NDP kinase from the hyperthermophilic archaebacterium Methanococcus jannaschii has been overexpressed in Escherichia coli and crystallized at 297 K using polyethylene glycol 4000 as precipitant. The crystal is hexagonal, belonging to the space group P63, with unit-cell parameters a = b = 72.89, c = 100.87 Å. The asymmetric unit contains two subunits of NDP kinase, with a corresponding crystal volume per protein mass (V(M)) of 2.38 Å3 Da-1 and a solvent content of 48.3%. Native X-ray diffraction data to 2.30 Å resolution have been collected using synchrotron X-rays.

Original languageEnglish
Pages (from-to)1485-1487
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number11
DOIs
Publication statusPublished - 2000 Nov 29
Externally publishedYes

Fingerprint

Methanocaldococcus
Nucleoside-Diphosphate Kinase
diphosphates
nucleosides
Archaea
Crystallization
X-Rays
crystallization
X rays
archaebacteria
Crystals
Synchrotrons
x rays
Nucleosides
X-Ray Diffraction
Escherichia coli
Escherichia
crystals
enzymes
glycols

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii : Overexpression, crystallization and preliminary X-ray crystallographic analysis. / Min, K.; Song, Hyun Kyu; Chang, C.; Jae Young Lee, Young Lee; Soo Hyun Eom, Hyun Eom; Kyeong Kyu Kim, Kyu Kim; Yeon Gyu Yu, Gyu Yu; Se Won Suh, Won Suh.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 56, No. 11, 29.11.2000, p. 1485-1487.

Research output: Contribution to journalArticle

Min, K. ; Song, Hyun Kyu ; Chang, C. ; Jae Young Lee, Young Lee ; Soo Hyun Eom, Hyun Eom ; Kyeong Kyu Kim, Kyu Kim ; Yeon Gyu Yu, Gyu Yu ; Se Won Suh, Won Suh. / Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii : Overexpression, crystallization and preliminary X-ray crystallographic analysis. In: Acta Crystallographica Section D: Biological Crystallography. 2000 ; Vol. 56, No. 11. pp. 1485-1487.
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