Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii: Overexpression, crystallization and preliminary X-ray crystallographic analysis

K. Min, Hyun Kyu Song, C. Chang, Young Lee Jae Young Lee, Hyun Eom Soo Hyun Eom, Kyu Kim Kyeong Kyu Kim, Gyu Yu Yeon Gyu Yu, Won Suh Se Won Suh

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Nucleoside diphosphate (NDP) kinase is a key enzyme in maintaining cellular pools of all nucleoside triphosphates. NDP kinase from the hyperthermophilic archaebacterium Methanococcus jannaschii has been overexpressed in Escherichia coli and crystallized at 297 K using polyethylene glycol 4000 as precipitant. The crystal is hexagonal, belonging to the space group P63, with unit-cell parameters a = b = 72.89, c = 100.87 Å. The asymmetric unit contains two subunits of NDP kinase, with a corresponding crystal volume per protein mass (V(M)) of 2.38 Å3 Da-1 and a solvent content of 48.3%. Native X-ray diffraction data to 2.30 Å resolution have been collected using synchrotron X-rays.

Original languageEnglish
Pages (from-to)1485-1487
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Issue number11
Publication statusPublished - 2000 Nov 29
Externally publishedYes


ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

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