Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones

D. N. Foster, D. Galehouse, T. Giordano, B. Min, I. C. Lamb, D. A. Porter, K. J. Intehar, W. L. Bacon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalJournal of Molecular Endocrinology
Volume8
Issue number1
DOIs
Publication statusPublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones'. Together they form a unique fingerprint.

  • Cite this

    Foster, D. N., Galehouse, D., Giordano, T., Min, B., Lamb, I. C., Porter, D. A., Intehar, K. J., & Bacon, W. L. (1992). Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones. Journal of Molecular Endocrinology, 8(1), 21-27. https://doi.org/10.1677/jme.0.0080021