Abstract
Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.
Original language | English |
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Pages (from-to) | 21-27 |
Number of pages | 7 |
Journal | Journal of Molecular Endocrinology |
Volume | 8 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1992 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Endocrinology