Abstract
Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.
| Original language | English |
|---|---|
| Pages (from-to) | 21-27 |
| Number of pages | 7 |
| Journal | Journal of Molecular Endocrinology |
| Volume | 8 |
| Issue number | 1 |
| Publication status | Published - 1992 Jan 1 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Endocrinology
Cite this
Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones. / Foster, D. N.; Galehouse, D.; Giordano, T.; Min, Bon Hong; Lamb, I. C.; Porter, D. A.; Intehar, K. J.; Bacon, W. L.
In: Journal of Molecular Endocrinology, Vol. 8, No. 1, 01.01.1992, p. 21-27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones
AU - Foster, D. N.
AU - Galehouse, D.
AU - Giordano, T.
AU - Min, Bon Hong
AU - Lamb, I. C.
AU - Porter, D. A.
AU - Intehar, K. J.
AU - Bacon, W. L.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.
AB - Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.
UR - http://www.scopus.com/inward/record.url?scp=0026542429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026542429&partnerID=8YFLogxK
M3 - Article
C2 - 1543531
AN - SCOPUS:0026542429
VL - 8
SP - 21
EP - 27
JO - Journal of Molecular Endocrinology
JF - Journal of Molecular Endocrinology
SN - 0952-5041
IS - 1
ER -