Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones

D. N. Foster, D. Galehouse, T. Giordano, Bon Hong Min, I. C. Lamb, D. A. Porter, K. J. Intehar, W. L. Bacon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalJournal of Molecular Endocrinology
Volume8
Issue number1
Publication statusPublished - 1992 Jan 1
Externally publishedYes

Fingerprint

Pituitary Hormones
Chickens
Glycoproteins
Complementary DNA
Nucleotides
Clone Cells
Amino Acids
Poly A
5' Untranslated Regions
3' Untranslated Regions
Pituitary Gland
Gonadotropin-Releasing Hormone
Northern Blotting
Open Reading Frames
Libraries
Peptides

ASJC Scopus subject areas

  • Endocrinology

Cite this

Foster, D. N., Galehouse, D., Giordano, T., Min, B. H., Lamb, I. C., Porter, D. A., ... Bacon, W. L. (1992). Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones. Journal of Molecular Endocrinology, 8(1), 21-27.

Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones. / Foster, D. N.; Galehouse, D.; Giordano, T.; Min, Bon Hong; Lamb, I. C.; Porter, D. A.; Intehar, K. J.; Bacon, W. L.

In: Journal of Molecular Endocrinology, Vol. 8, No. 1, 01.01.1992, p. 21-27.

Research output: Contribution to journalArticle

Foster, DN, Galehouse, D, Giordano, T, Min, BH, Lamb, IC, Porter, DA, Intehar, KJ & Bacon, WL 1992, 'Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones', Journal of Molecular Endocrinology, vol. 8, no. 1, pp. 21-27.
Foster, D. N. ; Galehouse, D. ; Giordano, T. ; Min, Bon Hong ; Lamb, I. C. ; Porter, D. A. ; Intehar, K. J. ; Bacon, W. L. / Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones. In: Journal of Molecular Endocrinology. 1992 ; Vol. 8, No. 1. pp. 21-27.
@article{20580c16e73e45ba9e89b22f72a80e49,
title = "Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones",
abstract = "Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79{\%} homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.",
author = "Foster, {D. N.} and D. Galehouse and T. Giordano and Min, {Bon Hong} and Lamb, {I. C.} and Porter, {D. A.} and Intehar, {K. J.} and Bacon, {W. L.}",
year = "1992",
month = "1",
day = "1",
language = "English",
volume = "8",
pages = "21--27",
journal = "Journal of Molecular Endocrinology",
issn = "0952-5041",
publisher = "Society for Endocrinology",
number = "1",

}

TY - JOUR

T1 - Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones

AU - Foster, D. N.

AU - Galehouse, D.

AU - Giordano, T.

AU - Min, Bon Hong

AU - Lamb, I. C.

AU - Porter, D. A.

AU - Intehar, K. J.

AU - Bacon, W. L.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.

AB - Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.

UR - http://www.scopus.com/inward/record.url?scp=0026542429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026542429&partnerID=8YFLogxK

M3 - Article

C2 - 1543531

AN - SCOPUS:0026542429

VL - 8

SP - 21

EP - 27

JO - Journal of Molecular Endocrinology

JF - Journal of Molecular Endocrinology

SN - 0952-5041

IS - 1

ER -