Nutrient removal from hydroponic wastewater by a microbial consortium and a culture of Paracercomonas saepenatans

Ju Yeon Lee, Arifur Rahman, Juliana Behrens, Conor Brennan, Baknoon Ham, Hyung Seok Kim, Chu Won Nho, Seong Taek Yun, Hossain Azam, Man Jae Kwon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The potential of microbial processes for removal of major nutrients (e.g., N, P) and inorganic cations (e.g., Ca2+, Mg2+, and Fe2+) from hydroponic systems was investigated. Microbial consortium- and axenic culture-based experiments were conducted in a waste nutrient solution (WNS). A microbial consortium grown in the WNS and selected microalgae species of Paracercomonas saepenatans were inoculated in two different synthetic media (Bold's Basal Medium (BBM) and synthetic WNS) in batch systems, and the microbial growth characteristics and the rate and extent of nutrient removal were determined for each system. No toxicity or growth inhibition was observed during microbial growth in either media. Both the waste-nutrient-grown microbial consortium and Paracercomonas saepenatans can be grown effectively in BBM and WNS, and both remove most ions from both media (e.g., >99% removal of NO3 and 41–100% removal of PO43−) within 16 days. Significant nutrient removal was observed during the growth phase of the microbial communities (4–10 days period), indicating major nutrient utilization for microbial growth as well as chemical mineral precipitation. Furthermore, MINEQL + 4.6 modeling showed higher PO43− removal in WNS during microbial growth (compared to BBM) due to precipitation of phosphate minerals (e.g., hydroxyapatite, vivianite). The dominant microbial species in both systems were also identified. DNA sequencing showed that Vorticella (58%) and Scenedesmus (33%) in WNS and Scenedesmus (89%) in BBM were the predominant species. This study demonstrates the potential application of microbial consortium (predominantly algae and protozoan)-based treatment techniques for hydroponic systems.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
JournalNew Biotechnology
Volume41
DOIs
Publication statusPublished - 2018 Mar 25

Fingerprint

Microbial Consortia
Hydroponics
Waste Water
Nutrients
Wastewater
Food
Growth
Scenedesmus
Minerals
Chemical Precipitation
Axenic Culture
Microalgae
Phosphate minerals
Durapatite
DNA Sequence Analysis
Algae
Cations
Hydroxyapatite
Toxicity
Phosphates

Keywords

  • Algae
  • Hydroponic system
  • Microbial community
  • Mineral precipitation
  • Nitrogen and phosphorous uptake

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Biology

Cite this

Nutrient removal from hydroponic wastewater by a microbial consortium and a culture of Paracercomonas saepenatans. / Lee, Ju Yeon; Rahman, Arifur; Behrens, Juliana; Brennan, Conor; Ham, Baknoon; Kim, Hyung Seok; Nho, Chu Won; Yun, Seong Taek; Azam, Hossain; Kwon, Man Jae.

In: New Biotechnology, Vol. 41, 25.03.2018, p. 15-24.

Research output: Contribution to journalArticle

Lee, Ju Yeon ; Rahman, Arifur ; Behrens, Juliana ; Brennan, Conor ; Ham, Baknoon ; Kim, Hyung Seok ; Nho, Chu Won ; Yun, Seong Taek ; Azam, Hossain ; Kwon, Man Jae. / Nutrient removal from hydroponic wastewater by a microbial consortium and a culture of Paracercomonas saepenatans. In: New Biotechnology. 2018 ; Vol. 41. pp. 15-24.
@article{170e08ac8fc849b88ede197cec59ca86,
title = "Nutrient removal from hydroponic wastewater by a microbial consortium and a culture of Paracercomonas saepenatans",
abstract = "The potential of microbial processes for removal of major nutrients (e.g., N, P) and inorganic cations (e.g., Ca2+, Mg2+, and Fe2+) from hydroponic systems was investigated. Microbial consortium- and axenic culture-based experiments were conducted in a waste nutrient solution (WNS). A microbial consortium grown in the WNS and selected microalgae species of Paracercomonas saepenatans were inoculated in two different synthetic media (Bold's Basal Medium (BBM) and synthetic WNS) in batch systems, and the microbial growth characteristics and the rate and extent of nutrient removal were determined for each system. No toxicity or growth inhibition was observed during microbial growth in either media. Both the waste-nutrient-grown microbial consortium and Paracercomonas saepenatans can be grown effectively in BBM and WNS, and both remove most ions from both media (e.g., >99{\%} removal of NO3− and 41–100{\%} removal of PO43−) within 16 days. Significant nutrient removal was observed during the growth phase of the microbial communities (4–10 days period), indicating major nutrient utilization for microbial growth as well as chemical mineral precipitation. Furthermore, MINEQL + 4.6 modeling showed higher PO43− removal in WNS during microbial growth (compared to BBM) due to precipitation of phosphate minerals (e.g., hydroxyapatite, vivianite). The dominant microbial species in both systems were also identified. DNA sequencing showed that Vorticella (58{\%}) and Scenedesmus (33{\%}) in WNS and Scenedesmus (89{\%}) in BBM were the predominant species. This study demonstrates the potential application of microbial consortium (predominantly algae and protozoan)-based treatment techniques for hydroponic systems.",
keywords = "Algae, Hydroponic system, Microbial community, Mineral precipitation, Nitrogen and phosphorous uptake",
author = "Lee, {Ju Yeon} and Arifur Rahman and Juliana Behrens and Conor Brennan and Baknoon Ham and Kim, {Hyung Seok} and Nho, {Chu Won} and Yun, {Seong Taek} and Hossain Azam and Kwon, {Man Jae}",
year = "2018",
month = "3",
day = "25",
doi = "10.1016/j.nbt.2017.11.003",
language = "English",
volume = "41",
pages = "15--24",
journal = "New Biotechnology",
issn = "1871-6784",
publisher = "Elsevier",

}

TY - JOUR

T1 - Nutrient removal from hydroponic wastewater by a microbial consortium and a culture of Paracercomonas saepenatans

AU - Lee, Ju Yeon

AU - Rahman, Arifur

AU - Behrens, Juliana

AU - Brennan, Conor

AU - Ham, Baknoon

AU - Kim, Hyung Seok

AU - Nho, Chu Won

AU - Yun, Seong Taek

AU - Azam, Hossain

AU - Kwon, Man Jae

PY - 2018/3/25

Y1 - 2018/3/25

N2 - The potential of microbial processes for removal of major nutrients (e.g., N, P) and inorganic cations (e.g., Ca2+, Mg2+, and Fe2+) from hydroponic systems was investigated. Microbial consortium- and axenic culture-based experiments were conducted in a waste nutrient solution (WNS). A microbial consortium grown in the WNS and selected microalgae species of Paracercomonas saepenatans were inoculated in two different synthetic media (Bold's Basal Medium (BBM) and synthetic WNS) in batch systems, and the microbial growth characteristics and the rate and extent of nutrient removal were determined for each system. No toxicity or growth inhibition was observed during microbial growth in either media. Both the waste-nutrient-grown microbial consortium and Paracercomonas saepenatans can be grown effectively in BBM and WNS, and both remove most ions from both media (e.g., >99% removal of NO3− and 41–100% removal of PO43−) within 16 days. Significant nutrient removal was observed during the growth phase of the microbial communities (4–10 days period), indicating major nutrient utilization for microbial growth as well as chemical mineral precipitation. Furthermore, MINEQL + 4.6 modeling showed higher PO43− removal in WNS during microbial growth (compared to BBM) due to precipitation of phosphate minerals (e.g., hydroxyapatite, vivianite). The dominant microbial species in both systems were also identified. DNA sequencing showed that Vorticella (58%) and Scenedesmus (33%) in WNS and Scenedesmus (89%) in BBM were the predominant species. This study demonstrates the potential application of microbial consortium (predominantly algae and protozoan)-based treatment techniques for hydroponic systems.

AB - The potential of microbial processes for removal of major nutrients (e.g., N, P) and inorganic cations (e.g., Ca2+, Mg2+, and Fe2+) from hydroponic systems was investigated. Microbial consortium- and axenic culture-based experiments were conducted in a waste nutrient solution (WNS). A microbial consortium grown in the WNS and selected microalgae species of Paracercomonas saepenatans were inoculated in two different synthetic media (Bold's Basal Medium (BBM) and synthetic WNS) in batch systems, and the microbial growth characteristics and the rate and extent of nutrient removal were determined for each system. No toxicity or growth inhibition was observed during microbial growth in either media. Both the waste-nutrient-grown microbial consortium and Paracercomonas saepenatans can be grown effectively in BBM and WNS, and both remove most ions from both media (e.g., >99% removal of NO3− and 41–100% removal of PO43−) within 16 days. Significant nutrient removal was observed during the growth phase of the microbial communities (4–10 days period), indicating major nutrient utilization for microbial growth as well as chemical mineral precipitation. Furthermore, MINEQL + 4.6 modeling showed higher PO43− removal in WNS during microbial growth (compared to BBM) due to precipitation of phosphate minerals (e.g., hydroxyapatite, vivianite). The dominant microbial species in both systems were also identified. DNA sequencing showed that Vorticella (58%) and Scenedesmus (33%) in WNS and Scenedesmus (89%) in BBM were the predominant species. This study demonstrates the potential application of microbial consortium (predominantly algae and protozoan)-based treatment techniques for hydroponic systems.

KW - Algae

KW - Hydroponic system

KW - Microbial community

KW - Mineral precipitation

KW - Nitrogen and phosphorous uptake

UR - http://www.scopus.com/inward/record.url?scp=85034812665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034812665&partnerID=8YFLogxK

U2 - 10.1016/j.nbt.2017.11.003

DO - 10.1016/j.nbt.2017.11.003

M3 - Article

C2 - 29174513

AN - SCOPUS:85034812665

VL - 41

SP - 15

EP - 24

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

ER -