O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1

Kihong Lim; Hyo Ihl Chang

doi:10.1016/j.febslet.2008.12.007

O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1

Kihong Lim, Hyo Ihl Chang

* Honorary professors

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Sp1 is a ubiquitous transcription factor that is modified by multiple O-linked N-acetylglucosamines (O-GlcNAc). Previously, O-GlcNAcylation of a specific site of Sp1 was shown to inhibit Sp1 transcriptional activity. Yet, how O-GlcNAc on other modification sites affects Sp1 function and how O-GlcNAcylation of Sp1 affects the transcriptional regulation of a target gene remains unknown. Here we show that O-GlcNAc within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1, and inhibits the cooperative activation of the U2 snRNA gene by Sp1 and Oct1. Structured summary: MINT-6803452: Sp1 (uniprotkb-P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti tag coimmunoprecipitation (MI:0007). MINT-6803426, MINT-6803438: Oct1 (uniprotkb:P14859) binds (MI:0407) to Sp1 (uniprotkb:P08047) by pull down (MI:0096). MINT-6803470, MINT-6803484: Sp1 (uniprotkb:P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti bait coimmunoprecipitation (MI:0006).

Original language	English
Pages (from-to)	512-520
Number of pages	9
Journal	FEBS Letters
Volume	583
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2008.12.007
Publication status	Published - 2009 Feb 4

Keywords

O-GlcNAc
Oct1
Sp1
U2 snRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.12.007

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title = "O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1",

abstract = "Sp1 is a ubiquitous transcription factor that is modified by multiple O-linked N-acetylglucosamines (O-GlcNAc). Previously, O-GlcNAcylation of a specific site of Sp1 was shown to inhibit Sp1 transcriptional activity. Yet, how O-GlcNAc on other modification sites affects Sp1 function and how O-GlcNAcylation of Sp1 affects the transcriptional regulation of a target gene remains unknown. Here we show that O-GlcNAc within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1, and inhibits the cooperative activation of the U2 snRNA gene by Sp1 and Oct1. Structured summary: MINT-6803452: Sp1 (uniprotkb-P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti tag coimmunoprecipitation (MI:0007). MINT-6803426, MINT-6803438: Oct1 (uniprotkb:P14859) binds (MI:0407) to Sp1 (uniprotkb:P08047) by pull down (MI:0096). MINT-6803470, MINT-6803484: Sp1 (uniprotkb:P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti bait coimmunoprecipitation (MI:0006).",

keywords = "O-GlcNAc, Oct1, Sp1, U2 snRNA",

author = "Kihong Lim and Chang, {Hyo Ihl}",

note = "Funding Information: We thank Dr. G. Westin (Ludwig Institute for Cancer Research, Uppsala, Sweden) for pET19b-Oct1. This work was supported by a Korea University Grant.",

year = "2009",

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doi = "10.1016/j.febslet.2008.12.007",

language = "English",

volume = "583",

pages = "512--520",

journal = "FEBS Letters",

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T1 - O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1

AU - Lim, Kihong

AU - Chang, Hyo Ihl

N1 - Funding Information: We thank Dr. G. Westin (Ludwig Institute for Cancer Research, Uppsala, Sweden) for pET19b-Oct1. This work was supported by a Korea University Grant.

PY - 2009/2/4

Y1 - 2009/2/4

N2 - Sp1 is a ubiquitous transcription factor that is modified by multiple O-linked N-acetylglucosamines (O-GlcNAc). Previously, O-GlcNAcylation of a specific site of Sp1 was shown to inhibit Sp1 transcriptional activity. Yet, how O-GlcNAc on other modification sites affects Sp1 function and how O-GlcNAcylation of Sp1 affects the transcriptional regulation of a target gene remains unknown. Here we show that O-GlcNAc within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1, and inhibits the cooperative activation of the U2 snRNA gene by Sp1 and Oct1. Structured summary: MINT-6803452: Sp1 (uniprotkb-P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti tag coimmunoprecipitation (MI:0007). MINT-6803426, MINT-6803438: Oct1 (uniprotkb:P14859) binds (MI:0407) to Sp1 (uniprotkb:P08047) by pull down (MI:0096). MINT-6803470, MINT-6803484: Sp1 (uniprotkb:P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti bait coimmunoprecipitation (MI:0006).

AB - Sp1 is a ubiquitous transcription factor that is modified by multiple O-linked N-acetylglucosamines (O-GlcNAc). Previously, O-GlcNAcylation of a specific site of Sp1 was shown to inhibit Sp1 transcriptional activity. Yet, how O-GlcNAc on other modification sites affects Sp1 function and how O-GlcNAcylation of Sp1 affects the transcriptional regulation of a target gene remains unknown. Here we show that O-GlcNAc within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1, and inhibits the cooperative activation of the U2 snRNA gene by Sp1 and Oct1. Structured summary: MINT-6803452: Sp1 (uniprotkb-P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti tag coimmunoprecipitation (MI:0007). MINT-6803426, MINT-6803438: Oct1 (uniprotkb:P14859) binds (MI:0407) to Sp1 (uniprotkb:P08047) by pull down (MI:0096). MINT-6803470, MINT-6803484: Sp1 (uniprotkb:P08047) physically interacts (MI:0218) with Oct1 (uniprotkb:P14859) by anti bait coimmunoprecipitation (MI:0006).

KW - O-GlcNAc

KW - Oct1

KW - Sp1

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JO - FEBS Letters

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ER -

O-GlcNAc modification of Sp1 inhibits the functional interaction between Sp1 and Oct1

Abstract

Keywords

ASJC Scopus subject areas

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