Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC

E. K. Kim, D. H. Shon, D. Ryu, J. W. Park, H. J. Hwang, Y. B. Kim

Research output: Contribution to journalArticlepeer-review

140 Citations (Scopus)


The occurrence of aflatoxin M1 (AFM1)in pasteurized milk and dairy products was investigated by using direct competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The recoveries of AFM1 from the samples spiked at levels between 5 and 500 pg/ml were 88.0–106.5% for pasteurized milk and 84.0–94.0% for yoghurt by ELISA. By HPLC, the recoveries were 103–120% for pasteurized milk and 87.0–93.0% for yoghurt. The limits of detection were found to be 2 pg/ml by ELISA and 10 pg/ml by HPLC. Among a total of 180 samples collected in Seoul, Korea, the incidence of AFM1 in pasteurized milk, infant formula, powdered milk and yoghurt was 76, 85, 75, and 83%, respectively, with a mean concentration of 18, 46, 200, and 29 pg/g, respectively, when determined by ELISA. These results obtained by ELISA were closely related to those by HPLC for AFM1 (r2 = 0.9783).

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalFood Additives and Contaminants
Issue number1
Publication statusPublished - 2000 Jan


  • Aflatoxin
  • Afm1
  • Assay
  • Chromatography
  • Elisa
  • Enzyme-linked
  • High
  • Hplc
  • Immunosorbent
  • Liquid
  • M1
  • Performance

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)
  • Chemistry(all)
  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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