Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria

Yeon Joon Park, Sun Young Park, Eun Jee Oh, Jung Jun Park, Kyo Young Lee, Gun-Jo Woo, Kyungwon Lee

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9%) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7%), TEM (46.3%), SHV (29.3%) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3%), specificity (82.7%), and positive (65.8%) and negative predictive values (98.3%).

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume51
Issue number4
DOIs
Publication statusPublished - 2005 Apr 1

Fingerprint

Citrobacter freundii
Enterobacter cloacae
Serratia marcescens
Cefotaxime
Polytetrafluoroethylene
Korea
Aztreonam
Ceftazidime
Microbial Sensitivity Tests
Cefuroxime
Clavulanic Acid
Sensitivity and Specificity
Genes

Keywords

  • Citrobacter freundii
  • Enterobacter cloacae
  • Extended-spectrum β-lactamase
  • Screening criteria
  • Serratia marcescens

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Occurrence of extended-spectrum β-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria. / Park, Yeon Joon; Park, Sun Young; Oh, Eun Jee; Park, Jung Jun; Lee, Kyo Young; Woo, Gun-Jo; Lee, Kyungwon.

In: Diagnostic Microbiology and Infectious Disease, Vol. 51, No. 4, 01.04.2005, p. 265-269.

Research output: Contribution to journalArticle

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abstract = "We assessed the occurrence and screening criterion for extended-spectrum β-lactamases (ESBLs) among AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens. The 413 isolates (158 E. cloacae, 126 C. freundii, and 129 S. marcescens) isolated from 11 clinical laboratories in Korea were investigated. ESBL production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZT), and cefepime (FEP) with or without clavulanic acid. One hundred seven isolates (25.9{\%}) were as ESBL producers. Of them, resistance was transferred by conjugation in 82 isolates. In transconjugants, structural genes for CTX-M (53.7{\%}), TEM (46.3{\%}), SHV (29.3{\%}) were found. To evaluate the ESBL screening minimum inhibitory concentration (MIC) criteria, MICs for cefuroxime, CAZ, CTX, AZT, and FEP were determined and cutoff value was selected using receiver operator characteristic curve. The FEP MIC ≥ 1 μg/mL had the highest sensitivity (95.3{\%}), specificity (82.7{\%}), and positive (65.8{\%}) and negative predictive values (98.3{\%}).",
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