Oceanimonas marisflavi sp. Nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium

Dong Wan Lee, Hanbyul Lee, Bong Oh Kwon, Jong Seong Khim, Un Hyuk Yim, Hongjae Park, Byeonghyeok Park, In-Geol Choi, Beom Seok Kim, Jae-Jin Kim

Research output: Contribution to journalArticle

Abstract

A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-Na3T, was isolated from sediment of Sinduri beach in Taean, Republic of Korea. Strain 102-Na3T grew optimally at 28–37 C, at pH 7.0–11.0 and in the presence of 1–3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain 102-Na3T joined the clade comprising the type strains of Oceanimonas species. Strain 102-Na3T exhibited 16S rRNA gene sequence similarity values of 98.8, 98.3 and 98.0 % to the type strains of Oceanimonas doudoroffii MBIC1298T, Oceanimonas baumannii GB6T and Oceanimonas smirnovii 31-13T, respectively. Strain 102-Na3T contained summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c), C16: 0 and C12: 0 as major fatty acids. The major quiENG was ubiquiENG-8. The polar lipids were composed of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 56.8 mol%. Strain 102-Na3T exhibited DNA–DNA relatedness values of 25.7, 21.7 and 14.8 % to the type strains of O. doudoroffii, O. baumannii and O. smirnovii, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain 102-Na3T is separated from recognized species of the genus Oceanimonas. On the basis of the data presented, strain 102-Na3T (=KCTC 62271T =JCM 32358T =DSM 106032T) is considered the type strain of a novel species of the genus Oceanimonas, for which the name Oceanimonas marisflavi sp. nov. is proposed.

Original languageEnglish
Article number002932
Pages (from-to)2990-2995
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number9
DOIs
Publication statusPublished - 2018 Sep 1

Fingerprint

Oceanimonas
Polycyclic Aromatic Hydrocarbons
polycyclic aromatic hydrocarbons
rRNA Genes
PAH
Bacteria
Lipids
Republic of Korea
Phosphatidylglycerols
bacterium
Cardiolipins
bacteria
Base Composition
lipid
Names
phylogenetics
Fatty Acids
gene
Oceanimonas baumannii
relatedness

Keywords

  • Beach sediment
  • Gammaproteobacteria
  • Oceanimonas
  • PAH degradation

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Oceanimonas marisflavi sp. Nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. / Lee, Dong Wan; Lee, Hanbyul; Kwon, Bong Oh; Khim, Jong Seong; Yim, Un Hyuk; Park, Hongjae; Park, Byeonghyeok; Choi, In-Geol; Kim, Beom Seok; Kim, Jae-Jin.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 9, 002932, 01.09.2018, p. 2990-2995.

Research output: Contribution to journalArticle

Lee, Dong Wan ; Lee, Hanbyul ; Kwon, Bong Oh ; Khim, Jong Seong ; Yim, Un Hyuk ; Park, Hongjae ; Park, Byeonghyeok ; Choi, In-Geol ; Kim, Beom Seok ; Kim, Jae-Jin. / Oceanimonas marisflavi sp. Nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 9. pp. 2990-2995.
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AU - Park, Hongjae

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N2 - A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-Na3T, was isolated from sediment of Sinduri beach in Taean, Republic of Korea. Strain 102-Na3T grew optimally at 28–37 C, at pH 7.0–11.0 and in the presence of 1–3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain 102-Na3T joined the clade comprising the type strains of Oceanimonas species. Strain 102-Na3T exhibited 16S rRNA gene sequence similarity values of 98.8, 98.3 and 98.0 % to the type strains of Oceanimonas doudoroffii MBIC1298T, Oceanimonas baumannii GB6T and Oceanimonas smirnovii 31-13T, respectively. Strain 102-Na3T contained summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c), C16: 0 and C12: 0 as major fatty acids. The major quiENG was ubiquiENG-8. The polar lipids were composed of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 56.8 mol%. Strain 102-Na3T exhibited DNA–DNA relatedness values of 25.7, 21.7 and 14.8 % to the type strains of O. doudoroffii, O. baumannii and O. smirnovii, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain 102-Na3T is separated from recognized species of the genus Oceanimonas. On the basis of the data presented, strain 102-Na3T (=KCTC 62271T =JCM 32358T =DSM 106032T) is considered the type strain of a novel species of the genus Oceanimonas, for which the name Oceanimonas marisflavi sp. nov. is proposed.

AB - A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-Na3T, was isolated from sediment of Sinduri beach in Taean, Republic of Korea. Strain 102-Na3T grew optimally at 28–37 C, at pH 7.0–11.0 and in the presence of 1–3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain 102-Na3T joined the clade comprising the type strains of Oceanimonas species. Strain 102-Na3T exhibited 16S rRNA gene sequence similarity values of 98.8, 98.3 and 98.0 % to the type strains of Oceanimonas doudoroffii MBIC1298T, Oceanimonas baumannii GB6T and Oceanimonas smirnovii 31-13T, respectively. Strain 102-Na3T contained summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), summed feature 8 (comprising C18: 1ω7c and/or C18: 1ω6c), C16: 0 and C12: 0 as major fatty acids. The major quiENG was ubiquiENG-8. The polar lipids were composed of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 56.8 mol%. Strain 102-Na3T exhibited DNA–DNA relatedness values of 25.7, 21.7 and 14.8 % to the type strains of O. doudoroffii, O. baumannii and O. smirnovii, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain 102-Na3T is separated from recognized species of the genus Oceanimonas. On the basis of the data presented, strain 102-Na3T (=KCTC 62271T =JCM 32358T =DSM 106032T) is considered the type strain of a novel species of the genus Oceanimonas, for which the name Oceanimonas marisflavi sp. nov. is proposed.

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