Ochratoxin A mediates cytotoxicity through the MAPK signaling pathway and alters intracellular homeostasis in bovine mammary epithelial cells

Jin Young Lee, Whasun Lim, Soomin Ryu, Jinyoung Kim, Gwonhwa Song

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ochratoxin A (OTA), a secondary metabolite of the genera Penicillium and Aspergillus, contaminates many types of food and causes apoptosis as well as immunosuppression in many animal species. However, a mechanistic analysis of OTA-mediated cytotoxicity in bovine mammary epithelial cells has not yet been performed. Hence, we investigated the effects of OTA on bovine mammary epithelial (MAC-T) cells using several mechanistic analyses. We report that OTA may induce cell cycle arrest and apoptosis via MAPK and JNK signaling pathways in MAC-T cells. Moreover, homeostasis of cellular components, such as that of the mitochondrial membrane, was disrupted by OTA, leading to a decrease in mitochondrial and cytosolic Ca2+ in MAC-T cells. In addition, we evaluated the effects of OTA on inflammatory responses and major tight junction regulators, such as occludin and claudin 3. In summation, we suggest that OTA contamination may adversely affect bovine mammary epithelial cells, leading to improper lactation and decreased milk quality. This article aims to improve the understanding of physiological mechanisms involved in lactation, in addition to providing a guideline for the stabilization of industrial milk production by countering exogenous contaminants in livestock.

Original languageEnglish
Pages (from-to)366-373
Number of pages8
JournalEnvironmental Pollution
Volume246
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

T-cells
Cytotoxicity
Breast
Homeostasis
Epithelial Cells
Cell death
Aspergillus
Metabolites
Agriculture
T-Lymphocytes
Lactation
Animals
Contamination
Stabilization
Cells
Impurities
Milk
Claudin-3
Membranes
Apoptosis

Keywords

  • Contaminant
  • Cow
  • Cytotoxicity
  • Mammary epithelial cell
  • Ochratoxin A

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Ochratoxin A mediates cytotoxicity through the MAPK signaling pathway and alters intracellular homeostasis in bovine mammary epithelial cells. / Lee, Jin Young; Lim, Whasun; Ryu, Soomin; Kim, Jinyoung; Song, Gwonhwa.

In: Environmental Pollution, Vol. 246, 01.03.2019, p. 366-373.

Research output: Contribution to journalArticle

@article{551e801f5af04000a9e8937f33251fe2,
title = "Ochratoxin A mediates cytotoxicity through the MAPK signaling pathway and alters intracellular homeostasis in bovine mammary epithelial cells",
abstract = "Ochratoxin A (OTA), a secondary metabolite of the genera Penicillium and Aspergillus, contaminates many types of food and causes apoptosis as well as immunosuppression in many animal species. However, a mechanistic analysis of OTA-mediated cytotoxicity in bovine mammary epithelial cells has not yet been performed. Hence, we investigated the effects of OTA on bovine mammary epithelial (MAC-T) cells using several mechanistic analyses. We report that OTA may induce cell cycle arrest and apoptosis via MAPK and JNK signaling pathways in MAC-T cells. Moreover, homeostasis of cellular components, such as that of the mitochondrial membrane, was disrupted by OTA, leading to a decrease in mitochondrial and cytosolic Ca2+ in MAC-T cells. In addition, we evaluated the effects of OTA on inflammatory responses and major tight junction regulators, such as occludin and claudin 3. In summation, we suggest that OTA contamination may adversely affect bovine mammary epithelial cells, leading to improper lactation and decreased milk quality. This article aims to improve the understanding of physiological mechanisms involved in lactation, in addition to providing a guideline for the stabilization of industrial milk production by countering exogenous contaminants in livestock.",
keywords = "Contaminant, Cow, Cytotoxicity, Mammary epithelial cell, Ochratoxin A",
author = "Lee, {Jin Young} and Whasun Lim and Soomin Ryu and Jinyoung Kim and Gwonhwa Song",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.envpol.2018.12.032",
language = "English",
volume = "246",
pages = "366--373",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Ochratoxin A mediates cytotoxicity through the MAPK signaling pathway and alters intracellular homeostasis in bovine mammary epithelial cells

AU - Lee, Jin Young

AU - Lim, Whasun

AU - Ryu, Soomin

AU - Kim, Jinyoung

AU - Song, Gwonhwa

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Ochratoxin A (OTA), a secondary metabolite of the genera Penicillium and Aspergillus, contaminates many types of food and causes apoptosis as well as immunosuppression in many animal species. However, a mechanistic analysis of OTA-mediated cytotoxicity in bovine mammary epithelial cells has not yet been performed. Hence, we investigated the effects of OTA on bovine mammary epithelial (MAC-T) cells using several mechanistic analyses. We report that OTA may induce cell cycle arrest and apoptosis via MAPK and JNK signaling pathways in MAC-T cells. Moreover, homeostasis of cellular components, such as that of the mitochondrial membrane, was disrupted by OTA, leading to a decrease in mitochondrial and cytosolic Ca2+ in MAC-T cells. In addition, we evaluated the effects of OTA on inflammatory responses and major tight junction regulators, such as occludin and claudin 3. In summation, we suggest that OTA contamination may adversely affect bovine mammary epithelial cells, leading to improper lactation and decreased milk quality. This article aims to improve the understanding of physiological mechanisms involved in lactation, in addition to providing a guideline for the stabilization of industrial milk production by countering exogenous contaminants in livestock.

AB - Ochratoxin A (OTA), a secondary metabolite of the genera Penicillium and Aspergillus, contaminates many types of food and causes apoptosis as well as immunosuppression in many animal species. However, a mechanistic analysis of OTA-mediated cytotoxicity in bovine mammary epithelial cells has not yet been performed. Hence, we investigated the effects of OTA on bovine mammary epithelial (MAC-T) cells using several mechanistic analyses. We report that OTA may induce cell cycle arrest and apoptosis via MAPK and JNK signaling pathways in MAC-T cells. Moreover, homeostasis of cellular components, such as that of the mitochondrial membrane, was disrupted by OTA, leading to a decrease in mitochondrial and cytosolic Ca2+ in MAC-T cells. In addition, we evaluated the effects of OTA on inflammatory responses and major tight junction regulators, such as occludin and claudin 3. In summation, we suggest that OTA contamination may adversely affect bovine mammary epithelial cells, leading to improper lactation and decreased milk quality. This article aims to improve the understanding of physiological mechanisms involved in lactation, in addition to providing a guideline for the stabilization of industrial milk production by countering exogenous contaminants in livestock.

KW - Contaminant

KW - Cow

KW - Cytotoxicity

KW - Mammary epithelial cell

KW - Ochratoxin A

UR - http://www.scopus.com/inward/record.url?scp=85059306133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059306133&partnerID=8YFLogxK

U2 - 10.1016/j.envpol.2018.12.032

DO - 10.1016/j.envpol.2018.12.032

M3 - Article

VL - 246

SP - 366

EP - 373

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

ER -