OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells

Sang Hun Choi, Jun Kyum Kim, Hee Young Jeon, Kiyoung Eun, Hyunggee Kim

Research output: Contribution to journalArticle

Abstract

OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies, especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the isoform of OCT4B, OCT4B-190 (OCT4B19kDa) was highly expressed in human glioblastoma stem cells and glioblastoma cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of OCT4B19kDa promoted colony formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed OCT4B at low levels. Thus, OCT4B19kDa may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalMolecules and cells
Volume42
Issue number2
DOIs
Publication statusPublished - 2019 Feb 28

Fingerprint

Glioblastoma
Protein Isoforms
Growth
Glioma
Octamer Transcription Factor-3
Induced Pluripotent Stem Cells
Alternative Splicing
Embryonic Stem Cells
Agar
Neoplasms
Cell Survival
Cytoplasm
Transcription Factors
Stem Cells

Keywords

  • anchorage-independent growth
  • cytoplasmic localization
  • glioblastoma
  • mechanical stress response
  • OCT4B

ASJC Scopus subject areas

  • Medicine(all)

Cite this

OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells. / Choi, Sang Hun; Kim, Jun Kyum; Jeon, Hee Young; Eun, Kiyoung; Kim, Hyunggee.

In: Molecules and cells, Vol. 42, No. 2, 28.02.2019, p. 135-142.

Research output: Contribution to journalArticle

Choi, Sang Hun ; Kim, Jun Kyum ; Jeon, Hee Young ; Eun, Kiyoung ; Kim, Hyunggee. / OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells. In: Molecules and cells. 2019 ; Vol. 42, No. 2. pp. 135-142.
@article{8e30cac65d854e13ba0fde1cd39dac6f,
title = "OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells",
abstract = "OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies, especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the isoform of OCT4B, OCT4B-190 (OCT4B19kDa) was highly expressed in human glioblastoma stem cells and glioblastoma cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of OCT4B19kDa promoted colony formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed OCT4B at low levels. Thus, OCT4B19kDa may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.",
keywords = "anchorage-independent growth, cytoplasmic localization, glioblastoma, mechanical stress response, OCT4B",
author = "Choi, {Sang Hun} and Kim, {Jun Kyum} and Jeon, {Hee Young} and Kiyoung Eun and Hyunggee Kim",
year = "2019",
month = "2",
day = "28",
doi = "10.14348/molcells.2018.0311",
language = "English",
volume = "42",
pages = "135--142",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "2",

}

TY - JOUR

T1 - OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells

AU - Choi, Sang Hun

AU - Kim, Jun Kyum

AU - Jeon, Hee Young

AU - Eun, Kiyoung

AU - Kim, Hyunggee

PY - 2019/2/28

Y1 - 2019/2/28

N2 - OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies, especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the isoform of OCT4B, OCT4B-190 (OCT4B19kDa) was highly expressed in human glioblastoma stem cells and glioblastoma cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of OCT4B19kDa promoted colony formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed OCT4B at low levels. Thus, OCT4B19kDa may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.

AB - OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies, especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the isoform of OCT4B, OCT4B-190 (OCT4B19kDa) was highly expressed in human glioblastoma stem cells and glioblastoma cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of OCT4B19kDa promoted colony formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed OCT4B at low levels. Thus, OCT4B19kDa may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.

KW - anchorage-independent growth

KW - cytoplasmic localization

KW - glioblastoma

KW - mechanical stress response

KW - OCT4B

UR - http://www.scopus.com/inward/record.url?scp=85062400334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062400334&partnerID=8YFLogxK

U2 - 10.14348/molcells.2018.0311

DO - 10.14348/molcells.2018.0311

M3 - Article

C2 - 30622231

AN - SCOPUS:85062400334

VL - 42

SP - 135

EP - 142

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 2

ER -