Octylphenol and nonylphenol affect decidualization of human endometrial stromal cells

Duck Sung Ko, Dong Ryul Lee, Haengseok Song, Jong-Hoon Kim, Chun Kyu Lim

Research output: Contribution to journalArticle

Abstract

This study investigated the effect of octylphenol (OP) and nonylphenol (NP) on decidualization of endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells were cultured for 12 days and decidualization was induced in vitro. The cells were exposed to OP (5 or 10 μM) or NP (5 μM) during in vitro decidualization. The expression levels of decidualization markers were analyzed using qRT-PCR. The amount of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1) was measured in medium using ELISA. The expression levels of PRL and IGFBP1 were significantly decreased by treatment with OP or NP. The amounts of PRL and IGFBP1 were also significantly decreased by treatment with OP or NP. The patterns of PRL and IGFBP1 secretion into medium were consistent with their expression patterns. The findings indicate that OP or NP may disrupt the decidualization of endometrial stromal cells and eventually affect the reproductive health of women.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalReproductive Toxicology
Volume89
DOIs
Publication statusPublished - 2019 Oct 1

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Keywords

  • Decidualization
  • Endocrine disrupting chemical
  • Endometrial stromal cell
  • Nonylphenol
  • Octylphenol

ASJC Scopus subject areas

  • Toxicology

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