Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells

Je Yong Choi, Hyun Mo Ryoo, Byung Heon Lee, In-San Kim, Rang Woon Park, Kun Young Sohn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Okadaic acid, a potent specific inhibitor of serine/threonine protein phosphatases type 1 and 2A, affects several gene expressions in various cells. To determine whether okadaic acid affects the expression of fibronectin in MC3T3-E1 cells, we measured mRNA level and synthesis of fibronectin by Northern blot hybridization and immunoprecipitation methods, respectively. Okadaic acid (10-50 ng/ml) increased both mRNA level and synthesis of fibronectin in a dose-dependent manner. The increase of fibronectin mRNA by okadaic acid was strongly attenuated by the inhibition of new protein synthesis. The results indicate that okadaic acid, inhibitor of protein phosphatases, increases fibronectin synthesis in MC3T3-E1 cells.

Original languageEnglish
Pages (from-to)871-876
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume39
Issue number5
Publication statusPublished - 1996 Sep 14
Externally publishedYes

Fingerprint

Okadaic Acid
Fibronectins
Messenger RNA
Protein Phosphatase 2
Phosphoprotein Phosphatases
Threonine
Immunoprecipitation
Gene expression
Northern Blotting
Serine
Gene Expression
Proteins

Keywords

  • Fibronectin
  • Okadaic acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Choi, J. Y., Ryoo, H. M., Lee, B. H., Kim, I-S., Park, R. W., & Sohn, K. Y. (1996). Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells. Biochemistry and Molecular Biology International, 39(5), 871-876.

Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells. / Choi, Je Yong; Ryoo, Hyun Mo; Lee, Byung Heon; Kim, In-San; Park, Rang Woon; Sohn, Kun Young.

In: Biochemistry and Molecular Biology International, Vol. 39, No. 5, 14.09.1996, p. 871-876.

Research output: Contribution to journalArticle

Choi, JY, Ryoo, HM, Lee, BH, Kim, I-S, Park, RW & Sohn, KY 1996, 'Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells', Biochemistry and Molecular Biology International, vol. 39, no. 5, pp. 871-876.
Choi, Je Yong ; Ryoo, Hyun Mo ; Lee, Byung Heon ; Kim, In-San ; Park, Rang Woon ; Sohn, Kun Young. / Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells. In: Biochemistry and Molecular Biology International. 1996 ; Vol. 39, No. 5. pp. 871-876.
@article{d4c73198cbed4c29ae20ee8ee5c298b6,
title = "Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells",
abstract = "Okadaic acid, a potent specific inhibitor of serine/threonine protein phosphatases type 1 and 2A, affects several gene expressions in various cells. To determine whether okadaic acid affects the expression of fibronectin in MC3T3-E1 cells, we measured mRNA level and synthesis of fibronectin by Northern blot hybridization and immunoprecipitation methods, respectively. Okadaic acid (10-50 ng/ml) increased both mRNA level and synthesis of fibronectin in a dose-dependent manner. The increase of fibronectin mRNA by okadaic acid was strongly attenuated by the inhibition of new protein synthesis. The results indicate that okadaic acid, inhibitor of protein phosphatases, increases fibronectin synthesis in MC3T3-E1 cells.",
keywords = "Fibronectin, Okadaic acid",
author = "Choi, {Je Yong} and Ryoo, {Hyun Mo} and Lee, {Byung Heon} and In-San Kim and Park, {Rang Woon} and Sohn, {Kun Young}",
year = "1996",
month = "9",
day = "14",
language = "English",
volume = "39",
pages = "871--876",
journal = "IUBMB Life",
issn = "1521-6543",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Okadaic acid increases fibronectin synthesis in MC3T3-E1 cells

AU - Choi, Je Yong

AU - Ryoo, Hyun Mo

AU - Lee, Byung Heon

AU - Kim, In-San

AU - Park, Rang Woon

AU - Sohn, Kun Young

PY - 1996/9/14

Y1 - 1996/9/14

N2 - Okadaic acid, a potent specific inhibitor of serine/threonine protein phosphatases type 1 and 2A, affects several gene expressions in various cells. To determine whether okadaic acid affects the expression of fibronectin in MC3T3-E1 cells, we measured mRNA level and synthesis of fibronectin by Northern blot hybridization and immunoprecipitation methods, respectively. Okadaic acid (10-50 ng/ml) increased both mRNA level and synthesis of fibronectin in a dose-dependent manner. The increase of fibronectin mRNA by okadaic acid was strongly attenuated by the inhibition of new protein synthesis. The results indicate that okadaic acid, inhibitor of protein phosphatases, increases fibronectin synthesis in MC3T3-E1 cells.

AB - Okadaic acid, a potent specific inhibitor of serine/threonine protein phosphatases type 1 and 2A, affects several gene expressions in various cells. To determine whether okadaic acid affects the expression of fibronectin in MC3T3-E1 cells, we measured mRNA level and synthesis of fibronectin by Northern blot hybridization and immunoprecipitation methods, respectively. Okadaic acid (10-50 ng/ml) increased both mRNA level and synthesis of fibronectin in a dose-dependent manner. The increase of fibronectin mRNA by okadaic acid was strongly attenuated by the inhibition of new protein synthesis. The results indicate that okadaic acid, inhibitor of protein phosphatases, increases fibronectin synthesis in MC3T3-E1 cells.

KW - Fibronectin

KW - Okadaic acid

UR - http://www.scopus.com/inward/record.url?scp=0029782585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029782585&partnerID=8YFLogxK

M3 - Article

C2 - 8866003

AN - SCOPUS:0029782585

VL - 39

SP - 871

EP - 876

JO - IUBMB Life

JF - IUBMB Life

SN - 1521-6543

IS - 5

ER -